Leukemia Stem Cells

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-0716-0809-8
Verlag: Springer US

Buch, Englisch, Band 2185, 479 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1121 g

Reihe: Methods in Molecular Biology

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Sánchez-García, Isidro

Cobaleda, César

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Naturwissenschaften Biowissenschaften Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Onkologie, Krebsforschung

Inhaltsverzeichnis

Introduction and Classification of Leukemias.- Leukemia Stem Cells: Concept and Implications.- Leukemia Stem Cell Drug Discovery.- Analysis and Isolation of Mouse Leukemic Stem Cells.- Mass Cytometry of Hematopoietic Cells.- PCR Technology to Identify Minimal Residual Disease.- Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies.- Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology).- Single-Cell Transcriptomic Analysis of Hematopoietic Cells.- In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics.- In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation.- Experimental Competitive Bone Marrow Transplant Assays.- Human T-ALL Xenografts.- An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts.- Leukemic Stem Cell Culture in Cytokine-Free Medium.- Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid.- Isolation, Culture, and Manipulation of Human Cord Blood Progenitors.- Lentiviral Transduction for Optimal LSC/HSC Manipulation.- Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes.- Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes.- Arrayed Molecular Barcoding of Leukemic Stem Cells.- In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis.- In Situ Hematopoietic Stem Cell Imaging.- A Genome-Editing System for Therapeutical Targeting of Stem Cells.- Method for the Generation of Induced Hematopoietic Stem Cells.- Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells.- High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells.- Bioinformatic Methods to Identify Mutational Signatures in Cancer.