Nanoparticles in Biology and Medicine

Methods and Protocols
2012
ISBN: 978-1-61779-952-5
Verlag: Humana Press

Buch, Englisch, Band 906, 555 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1266 g

Reihe: Methods in Molecular Biology

The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ‘70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery.  In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Soloviev, Mikhail

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Naturwissenschaften Biowissenschaften Proteinforschung
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Biotechnologie: Mikrotechnologie, Nanobiotechnologie
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie

Inhaltsverzeichnis

Part I: Noble Metal Nanoparticles: Synthesis and Applications 
1. Procedures for the Synthesis and Capping of Metal Nanoparticles            Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, and Miguel José-Yacamán
 
2. Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications            Yeon-Su Park 
3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis            Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, and Sangiliyandi Gurunathan 
4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays            Julian Bailes, Samantha Mayoss, Phil Teale, and Mikhail Soloviev
 
5. Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues            Sadaki Yokota 
6. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics            Joao Conde, Goncalo Doria, Jesus M. de la Fuente, and Pedro Viana Baptista
 
7. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins            Julian Bailes, Sara Gazi, Rositsa Ivanova, and Mikhail Soloviev 
Part II: Semiconductor Nanoparticles: Synthesis and Applications 
8. Synthesis of Semiconductor Nanoparticles            Xianfeng Chen and Peter J. Dobson
 
9. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature            Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, and Yoshinobu Baba
 
10. Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods
            Ana Sofia Miguel, Christopher Maycock, and Abel Oliva
 
11. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing            Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, and Paris A. Skourides
 
12. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis            Babu R. Prasad, Enda O’ Connell, Terry J. Smith, Valérie A. Gérard, Yurii K. Gun’ko, and Yury Rochev
 
13. The Use of Quantum Dots for Immunochemistry Applications            Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, and Carme Nogués
 
14. Quantum Dots for Labelling Live Cells            Yun Chen 
15. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots            Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, and Barbara J. Muller-Borer
 
16. The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry            Julian Bailes and Mikhail Soloviev 
Part III: Magnetic Nanoparticles: Synthesis and Applications 
17. Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging            Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen, and Ning Gu
 
18. Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging            Jasmin, Ana Luiza Machado Torres, Linda Jelicks, Antonio Carlos Campos de Carvalho, David C. Spray, and Rosalia Mendez-Otero
 
19. Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry            Maik Liebl and  Dietmar Eberbeck 
20. Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants            Alejandro Pérez-de-Luque, Zuny Cifuentes, Clara Marquina, Jesús M. de la Fuente, and M. Ricardo Ibarra
 
Part IV: Organic, Polymeric, Biomimetic, and Biocompatible Nanoparticles and Their Applications 
21. Synthesis of Biocompatible Gelatinated