Buch, Englisch, Band 560, 278 Seiten, Format (B × H): 198 mm x 266 mm, Gewicht: 897 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 560, 278 Seiten, Format (B × H): 198 mm x 266 mm, Gewicht: 897 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-934115-15-2
Verlag: Humana Press
Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In , leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Innere Medizin
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Diabetologie
Weitere Infos & Material
Selecting the “Right” Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research.- Nutritional Models of Type 2 Diabetes Mellitus.- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans.- The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans.- The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ?-Cell Lines.- In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function.- Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells.- Laser Capture Microdissection of Human Pancreatic ?-Cells and RNA Preparation for Gene Expression Profiling.- In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells.- The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes.- Pancreatic Remodeling: Beta-Cell Apoptosis, Proliferation and Neogenesis, and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size.- Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats.- Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas.- The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent.- Hyperinsulinemic–Euglycemic Clamp to Assess Insulin Sensitivity In Vivo.- Gene Expression Analysis in Diabetes Research.- Gene Expression Mining in Type 2 Diabetes Research.
