Basic Techniques in Molecular Biology

2000
ISBN: 978-3-540-66678-3
Verlag: Springer Berlin Heidelberg

Buch, Englisch, 434 Seiten, Format (B × H): 193 mm x 242 mm, Gewicht: 1690 g

Reihe: Springer Lab Manuals

This laboratory manual gives a thorough introduction to basic techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.
Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual.

Zielgruppe

Research

Autoren/Hrsg.

Surzycki, Stefan

Fachgebiete

• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tiergenetik, Reproduktion
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Immunologie
• Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1 General Aspects of DNA Isolation and Purification.- 2 Preparation of Genomic DNA from Animal Cells.- 3 Preparation of Genomic DNA from Plant Cells.- 4 Preparation of Genomic DNA from Bacteria.- 5 Isolation of Plasmid DNA.- 6 Isolation and Purification of RNA.- 7 Isolation of PolyA+ RNA.- 8 Agarose Gel Electrophoresis of DNA.- 9 Preparation of Probes for Hybridization.- 10 Nucleic Acid Hybridization. A Theoretical Consideration.- 11 DNA Transfer and Hybridization.- 12 Northern Transfer and Hybridization.- 13 DNA Cloning. General Consideration.- 14 DNA Cloning - Experimental Procedures.- 15 DNA Sequencing.- 16 PCR Analysis.