Tassieri Microrheology with Optical Tweezers

Principles and Applications
Erscheinungsjahr 2016
ISBN: 978-981-4669-19-1
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 328 Seiten

Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to µs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)!

Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies.

The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.

Autoren/Hrsg.

Tassieri, Manlio

Fachgebiete

• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Lasertechnologie, Holographie
• Technische Wissenschaften Maschinenbau | Werkstoffkunde Maschinenbau
• Naturwissenschaften Physik Physik Allgemein
• Technische Wissenschaften Technik Allgemein Technische Optik, Lasertechnologie

Inhaltsverzeichnis

Foreword; Miles J. Padgett

Introduction to "the nature of light"; R. Mike L. Evans

Geometrical optics; Alison Yao

Optical forces; Michael P. Lee and David B. Phillips

Optical tweezer configurations; Graham M. Gibson

Introduction to linear rheology; Manlio Tassieri

Statistical mechanics; Adrian Baule

Introduction to the most popular microrheology techniques; Aristeidis Papagiannopoulos

Microrheology with optical tweezers; Manlio Tassieri

Optical tweezers with microrheology; Richard W. Bowman

An appendix on evaluating the Fourier transform; R. Mike L. Evans