The Immunological Synapse - Part C, Volume 190 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand ?d T cells and evaluate their cytotoxicity.
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Weitere Infos & Material
Preface
Clément Thomas and Lorenzo Galluzzi
1. Analysis of immune synapse by STED imaging and 3D quantitative colocalization of lytic granule markers
Claudio Giraudo
2. Imaging the immune synapse: three-dimensional analysis of the immune synapse
Manuel Izquierdo
3. Image processing approaches for microtubule remodeling quantification at the immunological synapse
Andres Alcover, Marie Juzans, Celine Cuche and Vincenzo Di-Bartolo
4. Isolation and characterization of primary NK cells and their KIR2DL1+ population enrichment
Mira Barda-Saad
5. Chapter title to be confirmed
Johannes B. Huppa and Janett Göhring
6. Flow Cytometry Conjugate Formation Assay between Natural Killer (NK) Cells and Their Target Cells
Jacques Zimmer
7. Quantifying force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors
Katelyn Spillane, Anna Bajur and Hannah McArthur
8. Chapter title to be confirmed
Rose Thierry
9. Quantitative analysis of the B Cell immune synapse formation using imaging techniques
Isabel Yuseff
10. chapter title yet to confirmed
Rose Thierry
11. Expansion of ?d T cells and their use in in vitro cytotoxicity assays
Maria Alejandra Parigiani
12. A Comprehensive Guide to Studying the Immunological Synapse Using Imaging Flow Cytometry
Céline Hoffmann, Clément Thomas, Liza Filali, Andrea Michela Biolato and Max Krecke
Thomas, Clément
Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion.
