Thevis Mass Spectrometry in Sports Drug Testing

Characterization of Prohibited Substances and Doping Control Analytical Assays
1. Auflage 2010
ISBN: 978-0-470-62662-7
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 376 Seiten, E-Book

Reihe: Wiley-Interscience Series on Mass Spectrometry

Enables you to detect, identify, and characterize hundreds ofdrugs that may be used by athletes
Mass spectrometry has become essential to sports drug testing.This book examines both the principles of sports drug testing andthe use of mass spectrometry techniques and mass spectral data todetect, identify, and characterize hundreds of known and unknowndrugs that athletes may use to enhance their performance. Theauthor provides a detailed overview of the mass spectrometry ofnumerous classes of therapeutics and agents, various analyzers todetect low- and high-molecular weight drugs, as well as techniquesto discriminate between endogenously produced and syntheticallyderived compounds.
Mass Spectrometry in Sports Drug Testing begins with afull chapter dedicated to the history of sports drug testing. Next,the book provides the principles and techniques needed to maximizethe specificity and sensitivity of mass spectrometric assays,including:
* Detailed, step-by-step assays with sample preparation
* Discussion of both chromatographic separation and massspectrometric analysis
* Characterization of analytes in order to unequivocallyidentify banned substances
* Mass spectrometric behavior of low- and high-molecular weightanalytes
Throughout the book, descriptive examples illustrate theprinciples, advantages, and limitations of different assays.
Mass Spectrometry in Sports Drug Testing not only setsforth the role mass spectrometry plays in detecting drug use amongathletes, it also adds new insights into the health and ethicalissues of doping in sports.

Autoren/Hrsg.

Thevis, Mario

Inhaltsverzeichnis

Preface.
Acknowledgments.
1 History of Sports Drug Testing.
1.1 Historical Attempts of Artificial PerformanceEnhancement.
1.2 Background and Rationale of Doping Controls.
1.3 Early Detection Methods: Possibilities and Limitations ofAssays Without Mass Spectrometry.
1.4 Introduction of Mass Spectrometry to Doping ControlAnalysis.
References.
2. Mass Spectrometry and the List of Prohibited Substancesand Methods of Doping.
2.1 Criteria for the Mass Spectrometric Identification ofProhibited Compounds.
2.2 Modern Mass Spectrometers in Doping Controls: Advantages andDisadvantages of Available Techniques.
References.
3. Structure Characterization of Low Molecular Weight TargetAnalytes --Electron Ionization.
3.1 Stimulants.
3.2 Narcotics.
3.3 Anabolic Androgenic Steroids.
3.4 Selective Androgen Receptor Modulators (SARMs).
3.5 Diuretics.
3.6 beta2-Agonists.
3.7 beta-Receptor Blocking Agents.
3.8 Calcium-Channel Modulators (RYCALS).
3.9 Carbohydrate-Based Agents.
References.
4. Structure Characterization of Low Molecular Weight TargetAnalytes: Electrospray Ionization.
4.1 Stimulants.
4.2 Narcotics.
4.3 Anabolic Androgenic Steroids.
4.4 Selective Androgen Receptor Modulators (SARMs).
4.5 Diuretics.
4.6 beta2-Agonists.
4.7 Calcium-Channel Modulators (RYCALS).
4.8 Peroxisome-Proliferator Activated Receptor-delta(PPARdelta) And Adenosine Monophosphate Activated Protein Kinase(AMPK) Agonists.
4.9 Hypoxia-Inducible Factor (HIF)-Stabilizers And SirtuinActivators.
4.10 beta-Receptor Blocking Agents.
4.11 Glucuronic Acid and Sulfate Conjugates of TargetAnalytes.
References.
5. Structure Characterization of High Molecular Weight TargetAnalytes: Electrospray Ionization.
5.1 Human Chorionic Gonadotrophin (hCG).
5.2 Erythropoietins (EPO).
5.3 Synacthen.
5.4 Insulins.
5.5 Hemoglobin-Based Oxygen Carriers (HBOCs).
5.6 Human Growth Hormone (hGH).
5.7 Sermorelin (Geref).
5.8 Insulin-Like Growth Factor-1 (IGF-1).
5.9 Gonadorelin (LHRH).
References.
6. Modern Mass Spectrometry-Based Analytical Assays.
6.1 GC-MS and Isotope Ratio Mass Spectrometry.
6.2 LC-MS/MS.
References.
7. Limitations and Perspectives of Mass Spectrometry-BasedProcedures in Doping Control Analysis.
7.1 Recombinant Biomolecules.
7.2 Unknown Compounds.
7.3 Profiling of Urine and/or Blood.
7.4 Alternative Specimens.
References.
Index.

Über Autor(innen)

Mario Thevis, PhD, is Professor for Preventive Doping Research at the German Sport University Cologne. He has published more than 115 articles in peer-reviewed journals, mainly on mass spectrometry and sports drug testing. Dr. Tevis is often invited to national and international conferences as a speaker and keynote lecturer on mass spectrometry and doping controls.