Thompson Fluorescence Sensors and Biosensors

1. Auflage 2010
ISBN: 978-1-4200-2828-7
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 416 Seiten

Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts. The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations. The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.

Zielgruppe

Analytical chemists, pharmaceutical scientists, biochemists, molecular and cell biologists, clinicians, developers of photonics for environmental use, and students and researchers in these areas.

Autoren/Hrsg.

Thompson, Richard B.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Technische Wissenschaften Elektronik | Nachrichtentechnik Elektronik Sensorik
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie

Inhaltsverzeichnis

Introduction; T. Vo-Dinh
Prospects for the De Novo Design of Nucleic Acid Biosensors; L. Yang and A.D. Ellington
Biosensors Based on Periplasmic Binding Proteins; B.V. Sharma, S.S. Shrestha, S.K. Deo, and S. Daunert
Molecular Beacon DNA Probes Based on Fluorescence Biosensing; G. Yao, M.S. John, and W. Tan
Fluorescence Resonance Energy Transfer-Based Sensors for Bioanalysis; G. Blagoi, N. Rosenzweig, and Z. Rosenzweig
Carbonic Anhydrase-Based Biosensing of Metal Ions: Issues and Future Prospects; R.B. Thompson, R.A. Bozym, M.L. Cramer, A.V. Stoddard, N.K. Westerberg, and C.A. Fierke
Metal-Enhanced Fluorescence Sensing; C.D. Geddes, K. Aslan, I. Gryczynski, J. Malicka, and J.R. Lakowicz
Subpicomolar Assays of Antibodies and DNA Using Phosphorescence Labels; A.P. Savitsky
Development of Fluorescent Dipyrrolylquinoxaline-Based Anion Sensors; P. Anzenbacher, Jr. and K. Jursíková
Lab-on-a-Chip and Fluorescence Sensing on the Microscale; H.N. Chang, A.V. Hatch, K.R. Hawkins, and P. Yager
The Array Biosensors; F.S. Ligler and C.R. Taitt
Planar Waveguide Biosensors for Point-of-Care Clinical and Molecular Diagnostics; J.N. Herron, H.-K. Wang, L. Tan, S.Z. Brown, A.H. Terry, J.D. Durtschi, E.M. Simon, M.E. Astill, R.S. Smith, and D.A. Christensen
Fluorescence-Based Sensors for Bioprocess Monitoring; L. Tolosa, Y. Kostov, and G. Rao
Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist; R.B. Thompson, C.J. Frederickson, C.A. Fierke, N.M. Westerberg, R.A. Bozym, M.L. Cramer, and M. Hershfinkel Index