Timson Schistosoma mansoni

Methods and Protocols
Erscheinungsjahr 2020
ISBN: 978-1-0716-0635-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2151, 265 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume details protocols for studying

Schistosoma mansoni

.  Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from

S. mansoni,

describes a drug screening, and how to test potential vaccine targets. Written in the highly successful

Methods in Molecular Biology

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, 

Schistosoma mansoni: Methods and Protocols

aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Timson, David J.

Inhaltsverzeichnis

Praziquantel – An Enigmatic, Yet Effective Drug.- Computer-aided Drug Design for the Identification of Novel Antischistosomal Compounds.- Immunofluorescent Localization of Proteins in
Schistosoma mansoni.- 
Sensitive Fluorescence
in situ
hybridization on Semi-thin Sections of Adult
Schistosoma mansoni
using DIG-labeled RNA Probes.- Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke
Schistosoma mansoni.- 
Labeling of the 
Schistosoma mansoni
 Tegument.- A Procedure for Analyzing the Proteomic Profile of
Schistosoma mansoni
Cercariae.- Proteomic Analysis of
Schistosoma mansoni
tegumental Proteins.- Measuring Histone Modifications in the Human Parasite
Schistosoma mansoni.- 
Step-by-Step Bioinformatics Analysis of
Schistosoma mansoni
Long Non-coding RNA Sequences.- Identification of Transposable Elements in 
Schistosoma mansoni.- 
Structural and Functional Characterization of
Schistosoma mansoni
Cathepsin B1.- Characterisation of 
Schistosoma mansoni
 dihydrofolate Reductase (DHFR).- Characterisation of
Schistosoma mansoni
Glutamate-Gated Chloride Channels.- A Modified ELISA Method to Evaluate the Interaction of 
Schistosoma mansoni
 Proteins with Plasminogen.- Biophysical analysis of 
Schistosoma mansoni
 septins.- Crystallographic Studies of Triosephosphate Lsomerase from
Schistosoma mansoni.- 
Luminescence-based, Low- and medium-throughput Assays for Drug Screening in
Schistosoma mansoni
Larval Stage.- Testing of
Schistosoma mansoni
Vaccine Targets.