Timson Schistosoma mansoni

Methods and Protocols
Erscheinungsjahr 2020
ISBN: 978-1-0716-0635-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2151, 265 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume details protocols for studying Schistosoma mansoni .  Chapters guide readers through reviews on current drugs and drug discovery, methods to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from S. mansoni, describes a drug screening, and how to test potential vaccine targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,  Schistosoma mansoni: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Timson, David J.

Inhaltsverzeichnis

Praziquantel – An Enigmatic, Yet Effective Drug.- Computer-aided Drug Design for the Identification of Novel Antischistosomal Compounds.- Immunofluorescent Localization of Proteins in Schistosoma mansoni.-  Sensitive Fluorescence in situ hybridization on Semi-thin Sections of Adult Schistosoma mansoni using DIG-labeled RNA Probes.- Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni.-  Labeling of the  Schistosoma mansoni  Tegument.- A Procedure for Analyzing the Proteomic Profile of Schistosoma mansoni Cercariae.- Proteomic Analysis of Schistosoma mansoni tegumental Proteins.- Measuring Histone Modifications in the Human Parasite Schistosoma mansoni.-  Step-by-Step Bioinformatics Analysis of Schistosoma mansoni Long Non-coding RNA Sequences.- Identification of Transposable Elements in  Schistosoma mansoni.-  Structural and Functional Characterization of Schistosoma mansoni Cathepsin B1.- Characterisation of  Schistosoma mansoni  dihydrofolate Reductase (DHFR).- Characterisation of Schistosoma mansoni Glutamate-Gated Chloride Channels.- A Modified ELISA Method to Evaluate the Interaction of  Schistosoma mansoni  Proteins with Plasminogen.- Biophysical analysis of  Schistosoma mansoni  septins.- Crystallographic Studies of Triosephosphate Lsomerase from Schistosoma mansoni.-  Luminescence-based, Low- and medium-throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage.- Testing of Schistosoma mansoni Vaccine Targets.