Stem Cell Renewal and Cell-Cell Communication

Methods and Protocols
2. Auflage 2021
ISBN: 978-1-0716-1572-0
Verlag: Springer US

Buch, Englisch, Band 2346, 244 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 489 g

Reihe: Methods in Molecular Biology

This detailed book brings together a new set of protocols to arm cell biologists with techniques that are currently being used in a number of well-established laboratories around the world. The contents represent the great strides made in the field of cell-cell communications with respect to the identification and characterization of key components of the communication apparatus, assembly and maintenance of the communications structures, and concomitantly their roles in not only tissue formation and maintenance but also regeneration and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols, Second Edition serves as an ideal guide for experts and newcomers alike seeking to increase our understanding of the crucial biological and physiological roles of cell-cell communications in tissue function and organismal integrity.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Turksen, Kursad

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

- Inference of Ligand–Receptor Pairs from Single-Cell Transcriptomics Data. - Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells. - Interactions of Hematopoietic Stem Cells with Bone Marrow Niche. - Ex Vivo Modeling of Hematopoietic Stem Cell Homing to the Fetal Liver. - Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc. - A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival. - Calcium Fluorescence Recordings from Neuroepithelial Stem Cells. - Ultrastructural Analysis of Cell–Cell Interactions in Drosophila Ovary. - TIRF Microscopy as a Tool to Determine Exosome Composition. - Rapid Visualization of Intracellular Vesicle Events During Synaptic Stimulation. - Monitoring of Active Notch Signaling in Mouse Bladder Urothelium. - Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing. - Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approach to Examine Cell–Cell Communication. - Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins. - Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR. - Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells. - Fluorescent Labeling of Connexin with As Complex and X-Y Coordinate Registration of Target Single Cells Based on a Triangle Standard Chip for the Image Analysis of Gap Junctional Communication. - Chemical and Voltage Gating of Gap Junction Channels Expressed in Xenopus Oocytes. - The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching. - Flow Cytometry Evaluation of Gap Junction-Mediated Intercellular Communication Between Cytotoxic T Cells and Target Tumor Cells.