Van Dyke / Woodfork Luminescence Biotechnology

Instruments and Applications
Erscheinungsjahr 2010
ISBN: 978-1-4200-4180-4
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 624 Seiten

The dangers and drawbacks inherent in radioactivity-based methods along with a demonstrated and dramatic increase in sensitivity have precipitated a major shift towards luminescence measurements and visualization techniques. Their use has now spread even to traditional clinical environments, and their applications have grown from clinical assays to DNA sequencing, antioxidant detection, and high-throughput screening.

Luminescence Biotechnology: Instruments and Applications furnishes a thorough w review of the principles and applications of luminescence. With a consistent focus on practical considerations, contributions from a team of internationally acclaimed authors take you from the fundamentals of the different luminescence-based assay systems, calculation methods, and instruments through the spectrum of applications and latest research advances. Topics include gene and protein assays, oxidative stress and tissue aging, applications of luminescent microspheres, and proton image analysis.

This book clearly identifies the advantages of luminescence over other assay techniques, discusses its potential pitfalls, and illustrates the broad range of its utility. Whether you are a newcomer to the field or a seasoned professional, this book provides a wealth of information that will bring you quickly up to date on the technology, recent research developments, and cutting-edge applications.

Zielgruppe

Biotechnologists, biologists, chemists, forensic scientists, immunologists, microscopists, and toxicologists

Autoren/Hrsg.

Van Dyke, Knox

Van Dyke, Christopher

Woodfork, Karen

Fachgebiete

• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

INTRODUCTION TO LUMINESCENCE ASSAY
Light Probes, K. Van Dyke and K. Woodfork
Instrumentation for the Measurement of Luminescence, K. Van Dyke and K. Woodfork
Calculations to Quantify Luminescent Measurements, C. Van Dyke
A Low Budget Luminometer for Sensitive Chemiluminescent Immunoassays, N. Porakishvili, J. Fordham, M. Charrel, P. Delves, T. Lund, and I Roitt
Appendix A: Functionality of Commercially Available Instruments for Luminescence, K. Van Dyke
Appendix B: Survey of Commercially Available Instruments for Luminescence, C. Van Dyke
LIGHT PROBES/CLINICAL ASSAY
Chemiluminescent n-Sulfonyl Acridinium-9-Carboxamides and their Application in Clinical Assay, M. Adamczyk and P. Mattingly
Bioluminescent Detection of Proteolytic Bond Cleavage by Using Recombitant Aequorin, S. Deo, J. Lewis, and S. Daunert
Development of New Enzyme Labels for Bioluminescent Enzyme Immunoassay, M. Maeda
Assay of Diversified Biomolecules with a Luminescent Conjugate Substrate, T. Sudharanl and A. Reddy
Hybrid Capture®: A System for Nucleic Acid Detection by Signal Amplification Technology, A. Lorincz and James Anthony
MOLECULAR BIOLOGICAL APPLICATIONS OF LUMINESCENCE
Chemiluminescent Technology: Optimization of Western Blot and ELISA's Utilizing a Horseradish Peroxidase Label in Automated Systems, K. Hines, K. Feather-Henigan, L. Li, I. Horneij, C. Clothier, G. Milosevich, B. Lipton, and To Brotcke
A Luminal/Indophenol Chemiluminescent Detection System for Western Immunoblots, A. Yakunin and P. Hallenbeck
Chemiluminescent Detection of Immobilized Nucleic Acids-From Southern Blots to Microarrays, J. Tonkinson, B. Parker, and M. Harvey
Differential Display Polymerase Chain Reaction (PCR) for Using Chemiluminescent Detection, G. An and R. Veltri
Non-Radioactive Labeled Amlified Fragment Length Polymorphism for Application in Forest Trees, B. Ziegenhagen, R. Brettschneider, V. Kuhlenkamp, and M. Fladung
Detection of Nucleic Acids Using Chemiluminescence: From Northerns to Southerns and Beyond, J. Jackson, B. Strachan, D. von Schack, and L. Sylvers
Luciferase Assay, a Powerful Tool to Determine Toxic Methalp-Induces NT-kB Activation, F. Chen, K. Van Dyke, V. Vallaythan, V. Catranova, and X. Shi
Pyrosequencing: A Bioluminometric Method of DNA Sequencing, M. Ronaghi, N. Pourmand, and V. Stolc
Pathway-Specific cDNA Array Using Luminescent Detection, K. Van Dyke
CELLULAR LUMINESCENCE
Application of Chemiluminescence in Phagocyte-Pathogen Interactions, S. Gomis, D. Godson, and A. Potter
L-023, an Ultrasensitive Reagent for Detecting Cellular Generation of Reactive Oxygen Species, I. Imada, E. Sato, Y. Ichimori, Y. Aramaki, and M. Inoue
Avoiding the Jaws of Using Lucigenin as a Chemiluminigenic Probe for Biological Superoxide: Determining When it's Safe to Go Back into the Water, M. Trush and Y. Li
Chemiluminescent Polymer Microspheres for Measuring Reactive Oxygen Species, S. Hosaka and T. Uchida
The Effect of Antibiotics on Phagocytic Function of Granulocytes, P. Hengster, T. Eberl, F. Allerberger, M. Kunc, M. Dierich, and R. Margreiter
in vitro CMIä: Rapid Assay for Measuring Cell-Mediated Immunity, J. Britz, P. Sottong, and R. Kowalski
Protective Effects of Native but not Oxidized HDL Against Pro-Inflammatory Respiratory Burst Activities of Polymorphonuclear Leukocytes Induced by Hypochlorited-Oxidized LDL, S. Lopprasch, J. Pietzsch, and J. Gräßler
Peroxynitrite-Based Luminol Luminescence of Macrophages and Enhancement of the Signal, K. Van Dyke, P. McConnell, M. Taylor, and L.Frost
OXIDATIVE STRESS
Luminescence Analysis of Oxidative States Induced by organ Transplantation, A. Lojek, and M. Ciz
Acceleration Of Tissue Aging In Chickens Caused By Oxidative Stress Using Allopurinol and Detected By Cellular Humoral Chemiluminescence, H. Klandorf,D. Rathore, M. Iqbal, X. Shi, M. Simoyi, and K. Van Dyke
Enhancement of Luminal-Dependent-Peroxynitrite Luminescence in Dishes and Tubes from Various Macrophages: Rat Alveolar Macrophages Apparently Display a New Oxidative Mechanism, K. Van Dyke, M. Taylor, D. McConnell, and M. Reasor
A Search to Detect Antioxidants Using Luminescence Useful for Treating Oxidative Stress, K. Van Dyke, P. McConnell, and M. Sacks
Luminol Luminescence Generated by a Novel Peroxynitrite Mechanism is Inhibited by Polyphenolic Plant Extracts: Nitroglycerin Tablets and Superoxide Generate Peroxynitrite, N. Agarwal and K. Van Dyke
Antioxidant Activity in Grape and other Fruit Extracts Inhibit Peroxynitrite-Dependent Oxidation as Measured by Luminescnece, K. Van Dyke, C. Ogle, and M. Reasor
Antioxidant Action of Acetaminophen Preventing Myocardial Injury Detected by Luminescence and other Modalities, G. Merrill
Luciferase Luminescence can be Used to Access Oxidative Damage to Plasmid DNA and its Prevention by Selected Fruit Extracts, K. Van Dyke, C. Ogle, and M. Reasor
LUMINESCENT IMAGING
Detection System Optimized for Low Light Chemiluminescent Imaging, M. Christenson
Applications of Bio- and Chemiluminescent Imaging in Analytical Biotechnology, A. Roda, M. Guardigli, P. Pasini, M. Musiani, and M. Baraldini
Quantification of the a3 Subunit of the Na+/K+=-ATPase in Cerebellar Purkinjie Neurons Using Luminescence, P. Biser, J. Kong, W. Fleming, and D. Taylor
The Influence of Plates on Luminescent Measurements, P. Hengster, T. Eberl, W. Mark, M. Kunc, and W. Steurer
Whole Body Bioluminescent Imaging for the Study of Animal Models of Human Bacterial Disease, K. Francis, D. Joh, S. Buirns, C. Gruber, C. Contag, and P. Contag
HIGH THROUGHPUT SCREENING
Resonance Energy Transfer as an Emerging Technique for Monitoring Protein-Protein Interaction in vivo: BRET vs FRET, Y. Xu, A. Kanauchi, D. Piston, and C. Johnson
Bret2TM The Power of Two: Efficient Energy Transfer from Renilla Luciferase of GFP@ to Measure Protein-Protein Interactions and Intracellular Signaling Events in Live Cells, P. Dionne, M. Caron, A. Labonté, K. Allen, B. Houle, E. Joly, S.C. Taylor, and L. Menard
High Throughput Screening of Arabidopsis Mutants with De-regulated Stress-Responsive Luciferase Gene Expression Using a CCD Camera, B. Lee, B. Stevenson, and J.-K. Zhu
NorthStarä HTS Workstation: A CCD-Based Integrated Platform for High-Throughput Screening, D. Boldt-Houle, Y. Yan, C. Olesen, A. Chiulli, B. D'Eon, B. Liu, J. Voyta, and I. Bronstein
Versatile, Fast, Multilabel Biochip Reader Using a CCD Camera, F. Berthold, B. Breirkopf, and K. Van Dyke