Visualizing RNA Dynamics in the Cell

Erscheinungsjahr 2016
ISBN: 978-0-12-802292-4
Verlag: William Andrew Publishing

Buch, Englisch, 384 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 820 g

Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Enzymologie

Inhaltsverzeichnis

1. RNA Imaging with Multiplexed Error Robust Fluorescence in Situ Hybridization
J.R. Moffitt and X. Zhuang
2. Imaging Single mRNA Dynamics in Live Neurons and Brains
H.C. Moon and H.Y. Park
3. Monitoring of Rna Dynamics in Living Cells Using Pum-Hd and Fluorescent Protein Reconstitution Technique
H. Yoshimura and T. Ozawa
4. Applications of Hairpin DNA Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells
S.R. Jackson, A.C. Wong, A.R. Travis, I.E. Catrina, D.P. Bratu, D.W. Wright and A. Jayagopal
5. In Vivo RNA Visualization in Plants Using MS2 Tagging
E.J. Peña and M. Heinlein
6. Trick: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals
J.M. Halstead, J.H. Wilbertz, F. Wippich, T. Lionnet, A. Ephrussi and  J.A. Chao
7. Fluctuation Analysis: Dissecting Transcriptional Kinetics with Signal Theory
A. Coulon and D.R. Larson
8. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters
J. Ray, I. Shin, M. Ilgu, L. Bendickson, V. Gupta, G.A. Kraus and M. Nilsen-Hamilton
9. A Method for Expressing and Imaging Abundant, Stable, Circular Rnas in Vivo Using Trna Splicing
C.A. Schmidt, J.J. Noto, G.S. Filonov and A.G. Matera
10. RNA-ID, A Powerful Tool for Identifying and Characterizing Regulatory Sequences
C.E. Brule, K.M. Dean and E.J. Grayhack
11. Fluorescent Protein-Based Quantification of Alternative Splicing of a Target Cassette Exon in Mammalian Cells
N.G. Gurskaya, D.B. Staroverov and K.A. Lukyanov
12. Iras: High-Throughput Identification of Novel Alternative Splicing Regulators
S. Zheng
13. Analysis of Nonsense-Mediated mRNA Decay at the Single Cell Level using Two Fluorescent Proteins
N.G. Gurskaya, A.P. Pereverzev, D.B. Staroverov, N.M. Markina and K.A. Lukyanov
14. Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells
J.L. Litke, M. You and S.R. Jaffrey