Vlak / de Gooijer / Tramper Insect Cell Cultures

Fundamental and Applied Aspects
1996
ISBN: 978-0-306-46850-6
Verlag: Springer Netherland
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2, 316 Seiten, eBook

Reihe: Current Applications of Cell Culture Engineering

Zielgruppe

Research

Autoren/Hrsg.

Vlak, Just M.

de Gooijer, Cornelis D.

Tramper, Johannes

Miltenburger, Herbert G.

Inhaltsverzeichnis

Insect Cell Lines.- Development and characterization of insect cell lines.- New approaches to insect tissue culture.- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene.- Insect cell physiology.- Insect cell cultivation: growth and kinetics.- Medium design for insect cell culture.- Viruses.- Baculovirus-insect cell interactions.- Replication patterns and cytopathology of cells infected with baculoviruses.- Construction of baculovirus recombinants.- Passage effect of virus infection in insect cells.- Post-translational modifications in insect cells.- Productivity of insect cells for recombinant proteins.- Chaperone and foldase coexpression in the baculovirus-insect cell expression system.- Engineering Aspects.- Shear sensitivity of insect cells.- Insect cell bioreactors.- Perfusion bioreactors for the production of recombinant proteins in insect cells.- Immobilization of insect cells.- Modelling baculovirus infection of insect cells in culture.- Scale up aspects of sparged insect-cell bioreactors.- Oxygen gradients in small and big sparged insect-cell bioreactors.- Downstream processing of insect cell cultures.- Applications.- Parvovirus diagnostics and vaccine production in insect cells.- Classical swine fever virus diagnostics and vaccine production in insect cells.- Production of multidomain complement glycoproteins in insect cells.- Economic and Regulatory Aspects.- Economics of baculovirus-insect cell production systems.- Safety aspects of insect cell culture.- Regulatory issues in the use of insect-cell culture.