Waksmundzka-Hajnos / Sherma High Performance Liquid Chromatography in Phytochemical Analysis

1. Auflage 2011
ISBN: 978-1-4200-9261-5
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: 0 - No protection

E-Book, Englisch, 996 Seiten

Reihe: Chromatographic Science Series

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field.

The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products.

Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.

Zielgruppe

Plant scientists, analytical chemists in the pharmaceutical/supplement industries and regulatory agencies, natural product chemists; undergraduate and graduate students in chemistry, biology, and plant science.

Autoren/Hrsg.

Waksmundzka-Hajnos, Monika

Sherma, Joseph

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie

Inhaltsverzeichnis

PART I
Overview of the Field of Column High Performance Liquid Chromatography in Phytochemical Analysis and the Structure of the Book, Monika Waksmundzka-Hajnos and Joseph Sherma
Herbal Drugs and the Role of Chromatographic Methods in Their Analysis, Ioanna Chinou
Plant Products in Nutrition and Dietary Supplements: Quality Control, Grazyna Zgórka
HPLC in Chemosystematics, Renate Spitaler and Christian Zidorn
Phytochemistry, Phytopharmacology, and the Biological Role of Plant Metabolites, Krystyna Skalicka-Wozniak, Michal Hanos, and Kazimierz Glowniak
Sample Preparation of Plant Material, Anna Oniszczuk and Anna Hawryl
Stationary Phases and Columns in Analysis of Primary and Secondary Metabolites, Fred Rabel
Separation of Nonionic Analytes: Reversed- and Normal-Phase HPLC, Miroslaw Hawryl and Wojciech Markowski
Separation of Ionic Analytes: Reversed-Phase, Ion-Pair, Ion-Exchange, and Ion-Exclusion HPLC, Monika Waksmundzka-Hajnos and Lukasz Ciesla
Gradient Elution and Computer-Assisted Method Development, Miroslaw Hawryl
LC-MS as a Method of Identification and Quantification of Plant Metabolites, Gunther Stecher, Robert Mayer, Thomas Ringer, Muhammad A. Hashir, Saowapak Kasemsook, Muhammad N. Qureshi, and Gunther K. Bonn
LC-NMR and Related Techniques for the Rapid Identification of Plant Metabolites, Jean-Luc Wolfender
Photodiode Array (PDA) and Other Detection Methods in HPLC of Plant Metabolites, Wojciech Markowski and Monika Waksmundzka-Hajnos
Quantitative Analysis—Method Validation—Quality Control, Pierre Masson
Confirmation of Chirality of Some Natural Products by the HPLC Method, Beata Polak
PART II
Primary Metabolites
HPLC of Carbohydrates, Angelika Koch, Simla Basar, and Rita Richter
HPLC of Plant Lipids, Marek Golebiowski, Monika Paszkiewicz, Lukasz Halinski, and Piotr Stepnowski
HPLC Analysis of Amino Acids, Peptides, and Proteins, Giuseppe Mennella, Antonietta D’Alessandro, and Gianluca Francese
Secondary Metabolites—Shikimic Acid Derivatives
Application of HPLC in the Analysis of Phenols, Phenolic Acids, and Tannins, Tuulia Hyotylainen and Maarit Kivilompolo
Application of HPLC in Coumarin Analyses, Alev Tosun and Petr Tomek
High Performance Liquid Chromatography of Flavonoids, Monika Waksmundzka-Hajnos, Anna Oniszczuk, Mieczyslaw Hajnos, and Tomasz Oniszczuk
HPLC of Lignans, Annika I. Smeds
Secondary Metabolites—Isoprenoids
HPLC of Mono- and Sesquiterpenes, Angelika Koch, Simla Basar, and Rita Richter
HPLC Analysis of Diterpenes, Michal L. Hajnos
High Performance Liquid Chromatography of Triterpenes (Including Saponins), Wieslaw Oleszek and Anna Stochmal
HPLC of Carotenoids, Lukasz Ciesla
HPLC of Steroids, Laurence Dinan, Juraj Harmatha, and René Lafont
HPLC of Iridoids, Rilka Taskova, Tetsuo Kokubun, and Kalina Alipieva
Secondary Metabolites—Amino Acid Derivatives
HPLC of Indole Alkaloids, Anna Petruczynik
HPLC of Isoquinoline Alkaloids, László Kursinszki, Hajnalka Hank, Ágnes Kéry, and Éva Szoke
HPLC of Tropane Alkaloids, Tomasz Mroczek
HPLC of Alkaloids from the Other Biosynthetic Groups, Jolanta Flieger
Secondary Metabolites—Compounds Derived from Acetogenine (Acetylocoenzyme A)
HPLC Analysis of Polyacetylenes, Lars P. Christensen and Kathrine B. Christensen
HPLC of Quinonoid Phytochemicals, Subhalakshmi Ghosh, Madhushree Das Sarma, and Banasri Hazra
Index

Über Autor(innen)

Monika Waksmundzka-Hajnos is a professor in the Department of Inorganic Chemistry at the Medical University of Lublin in Poland.
Joseph Sherma is the John D. and Frances H. Larkin Professor Emeritus of Chemistry in the Department of Chemistry at Lafayette College in Easton, Pennsylvania, USA.