Mixed-Mode Chromatography

Principles, Methods, and Applications
1. Auflage 2021
ISBN: 978-981-16-5487-9
Verlag: Springer Nature Singapore

Buch, Englisch, 482 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 744 g

The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.

Zielgruppe

Research

Autoren/Hrsg.

Wan, Qian-Hong

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie
• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Labormedizin

Inhaltsverzeichnis

Chapter 1.Historical Perspectives.- Chapter 2.Theoretical Background.- Chapter 3.Modes of Chromatographic Separation.- Chapter 4.Chromatographic Matrices.- Chapter 5.Ligand Design.- Chapter 6.Retention Mechanisms.- Chapter 7.Stationary Phases.- Chapter 8.Mobile Phases and Elution.- Chapter 9.Method Development.- Chapter 10.Applications in Biopharmaceutical Processing.

Über Autor(innen)

Qian-Hong Wan is a professor in the Pharmaceutical Analysis department, School of Pharmaceutical Science and Technology, Tianjin University. He obtained his Ph.D. from Edinburgh University in 1993 and completed his two post-doctoral training at University of Amsterdam and Nottingham University. His research is focused on modern separation and analysis technology.