Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification

1. Auflage 2016
ISBN: 978-3-662-53397-0
Verlag: Springer

Buch, Englisch, 96 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 363 g

Reihe: Springer Theses

This thesis focuses on the study of interactions between protein and peptides and their potential applications in cell imaging and nanoparticle surface modification. Drawing inspiration from naturally occurring coiled-coil binding pairs, it proposes a novel covalent peptide tag and probe system, based on the concept of “affinity guided covalent conjugation.” This newly established methodology provides complementary resolution to protein labeling, imaging and trafficking. By systematically investigating the coordination interaction between protein and quantum dots using various engineered protein ligands, this thesis proposes a general rule for protein self-assembly on the surface of quantum dots and reports a revolutionized nanobelt protein in accordance with this rule. It is an extraordinary example of interdisciplinary research, providing answers to real-life biological problems from a chemistry perspective.

Zielgruppe

Research

Autoren/Hrsg.

Wang, Jianpeng

Fachgebiete

• Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde Materialwissenschaft: Biomaterialien, Nanomaterialien, Kohlenstoff
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Technische Wissenschaften Technik Allgemein Nanotechnologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Biotechnologie: Mikrotechnologie, Nanobiotechnologie

Inhaltsverzeichnis

Introduction.- Biological Active Antifungal Peptides.- Protein Ligands Engineering.- Coiled Coil Binding Induced Covalent Cross-linking.- Cell Surface Receptor Labeling.- Summary and Conclusion.