Whitman / Sater Analysis of Growth Factor Signaling in Embryos

Erscheinungsjahr 2010
ISBN: 978-1-4200-0478-6
Verlag: Taylor & Francis
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 435 Seiten

Reihe: Methods in Signal Transduction Series

Developmental biologists have been driven to investigate growth factor signaling in embryos in order to understand the regulatory mechanisms underlying a given developmental process. Thus, it is critical to explore the technical methods and experimental designs for growth factor signaling in embryos. Focusing on specific pathways or pathway components, Analysis of Growth Factor Signaling in Embryos provides the methods and guidelines for experimental design to study major aspects of cell signaling in vertebrate embryos. The book covers a broad range of topics in signaling and a variety of current model organisms. Section I explores specific signaling pathways or pathway components. In this section, some chapters highlight the biochemistry of signaling pathways during development, which is often distinctive from that observed in cell culture systems. Section II discusses ionic regulatory mechanisms and the two chapters in Section III examine ways of investigating gene regulation in response to extracellular signals. Finally, Section IV addresses emerging strategies that facilitate integrated analyses of cell signaling in vivo in embryonic systems. Featuring contributions from expert researchers, Analysis of Growth Factor Signaling in Embryos will provide a foundation for further explorations of the cellular regulatory mechanisms governing vertebrate embryonic development.

Zielgruppe

Developmental biologists, cell biologists, toxicologists, biomedical researchers, and graduate students.

Autoren/Hrsg.

Whitman, Malcolm

Sater, Amy

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Embryologie

Inhaltsverzeichnis

SIGNALS AND PATHWAYS
Analysis of Canonical Wnt Signaling in Xenopus Embryos
Wilson K. Clements and David Kimelman
How to Assay Non-Canonical Wnt Signaling: A Critical Analysis
Michael Kühl and Randall T. Moon
Regulation of TGF-ß Family Activity by Proprotein Processing
Shailaja Sopory and Jan L. Christian
Analysis of MAP Kinase Pathways in Vertebrate Development
Amy K. Sater and Heithem M. El-Hodiri
Analysis of Retinoid Signalling in Embryos
Malcolm Maden
Wnt Signaling via the Rho Family of GTPases during Embryonic Development
Raymond Habas and Xi He
Phosphospecific Antibodies as Tools for the Study of Signal
Transduction during Development
Malcolm Whitman
IONIC SIGNALS
Dynamic Analysis of Calcium Signaling in Animal Development
Diane C. Slusarski
Strategies and Techniques for Investigation of Biophysical Signals in Patterning
D.S. Adams and M. Levin
TRANSCRIPTIONAL REGULATION OF TARGET GENES
Expression Profiling in Xenopus Embryos
Curtis Altmann
Chromatin Immunoprecipitation for in vivo Studies of
Transcriptional Regulation during Development
Daniel R. Buchholz, Bindu D. Paul, and Yun-Bo Shi
EMERGING STRATEGIES FOR THE ANALYSIS OF SIGNALING IN DEVELOPMENT
Chemical Biology in Zebrafish Vascular Development
Joanne Chan and Thomas M. Roberts
Investigating Gastrulation
Karen Symes
Bringing Small Molecule Regulation of Protein Activity to
Developmental Systems
Karen J. Liu, Jason E. Gestwicki, and Gerald R. Crabtree
Systems Analysis of Signaling Pathways
Gregory R. Hoffman, Kevin Brown, Adrian Salic, and Ethan Lee
INDEX