Alexander / Block Determination of the Size and Shape of Protein Molecules

A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Vol. 3
1. Auflage 2014
ISBN: 978-1-4832-2128-1
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 296 Seiten, Web PDF

Analytical Methods of Protein Chemistry, Volume 3: Determination of the Size and Shape of Protein Molecules provides information pertinent to the analysis and isolation of protein. This book deals with the measurement of the macromolecular properties of proteins. Organized into seven chapters, this volume begins with an overview of the theory and practice of the electron microscope to allow an understanding of the type of object that may be examined. This text then describes the methods of making protein molecules conform to such an ideal, which are the techniques of specimen preparation. Other chapters consider the determinations of osmotic pressures of proteins. This book discusses as well the experimental basis for the theory of the diffusion process in liquids. The final chapter deals with the technical problem characteristics of light-scattering. This book is a valuable resource for electron microscopists, protein chemists, biologists, physicist, physico-chemists, scientists, and research workers.

Autoren/Hrsg.

Alexander, P.

Block, R. J.

Inhaltsverzeichnis

1;Front Cover;1
2;Determination of the Size and Shape of Protein Molecules;4
3;Copyright Page;5
4;Table of Contents;8
5;PREFACE;10
6;CONTENTS LIST: VOLUME 1;11
7;CONTENTS LIST: VOLUME 2;11
8;CHAPTER 1. TECHNIQUES FOR THE ELECTRON MICROSCOPY OF PROTEINS;12
8.1;INTRODUCTION;14
8.2;THE ELECTRON MICROSCOPE;14
8.3;PREPARATION OF SUPPORTING FILMS;16
8.4;APPLICATION OF PROTEIN TO FILMS;22
8.5;SHADOWING;25
8.6;STAINING;27
8.7;REPLICA METHODS;29
8.8;SECTION METHODS;31
8.9;INTERPRETATION OF ELECTRON MICROGRAPHS;32
8.10;APPLICATIONS OF THE METHOD;33
8.11;REFERENCES;35
9;CHAPTER 2. OSMOTIC PRESSURE;36
9.1;INTRODUCTION;37
9.2;PRINCIPLE;39
9.3;PREPARATION OF MEMBRANES;46
9.4;OSMOMETERS;51
9.5;REFERENCES;68
10;CHAPTER 3. TRANSLATIONAL DIFFUSION METHODS IN PROTEIN CHEMISTRY;70
10.1;INTRODUCTION;71
10.2;DIFFERENTIAL EQUATIONS OF DIFFUSION;71
10.3;INFORMATION AVAILABLE FROM DIFFUSION STUDIES;73
10.4;EXPERIMENTAL METHODS FOR STUDYING LINEAR DIFFUSION;78
10.5;REFERENCES;128
11;CHAPTER 4. ULTRACENTRIFUGATION;136
11.1;INTRODUCTION;138
11.2;GENERAL PRINCIPLES OF ULTRACENTRIFUGATION;138
11.3;SEDIMENTATION VELOCITY ULTRACENTRIFUGATION;145
11.4;EQUILIBRIUM ULTRACENTRIFUGATION;162
11.5;THE DIFFERENTIAL EQUATION FOR ULTRACENTRIFUGATION;173
11.6;PARTIAL SPECIFIC VOLUME, SIZE ANDSHAPE OF PROTEIN MOLECULES;175
11.7;PRACTICAL DETAILS;179
11.8;REFERENCES;190
12;CHAPTER 5. VISCOSITY;196
12.1;INTRODUCTION;198
12.2;GENERAL THEORY;198
12.3;METHODS OF MEASURING VISCOSITY;209
12.4;CAPILLARY VISCOMETERS;212
12.5;THE DETERMINATION OF LIMITING VISCOSITY NUMBER;215
12.6;GENERAL COMMENTS;219
12.7;PARTIAL SPECIFIC VOLUME;224
12.8;APPLICATIONS;225
12.9;SUMMARY AND CONCLUSIONS;228
12.10;REFERENCES;229
13;CHAPTER 6. UNIMOLECULAR LAYERS IN PROTEIN ANALYSIS;234
13.1;INTRODUCTION;235
13.2;THEORY AND TECHNIQUES IN UNIMOLECULAR LAYER CHEMISTRY;237
13.3;DETERMINATION OF MOLECULAR WEIGHT;250
13.4;TWO-DIMENSIONAL VISCOSITY AND ELASTICITY;258
13.5;PROTEIN DIFFUSION COEFFICIENTS OBTAINED WITH MONOLAYER TECHNIQUES;259
13.6;REACTION OF PROTEINS WITH "ANIONIC" FILMS;263
13.7;SUMMARY;264
13.8;REFERENCES;265
14;CHAPTER 7. THE USE OF LIGHT-SCATTERING FOR THE MEASUREMENT OF THE MOLECULAR WEIGHT AND SIZE OF PROTEINS;268
14.1;INTRODUCTION;269
14.2;THEORY;271
14.3;THE PRACTICE OF LIGHT-SCATTERING;283
14.4;GLOSSARY;295
14.5;REFERENCES;296
15;AUTHOR INDEX;300
16;SUBJECT INDEX;306