Apobec Enzymes

Erscheinungsjahr 2025
ISBN: 978-0-443-31786-6
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Apobec Enzymes, Volume 713 in this series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Fluorescent shift assay for APOBECs RNA editing, Low Error Sequencing Methods to Detect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System, "Safe-Barcode" RNAseq assay for APOBECs RNA editing, DT40 cell system to characterize somatic hypermutation, CH12 cell system to assay AID activity on class switch recombination, Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System, and more.

Additional chapters cover Defining genome-wide mutagenic impact of APOBEC3 enzymes, APOBEC-induced mutational assay in yeast, Assays for APOBEC drug discovery, Biochemical assay for the identification of APOBECs inhibitors, An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA, Profiling rare C-to-U editing events via direct RNA sequencing, Global quantification of off-target activity by base editors, and so much more.

Autoren/Hrsg.

Weitere Mitwirkende

Papavasiliou, F. Nina

Pecori, Riccardo

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

Section 1: Assays to characterize DNA or RNA editing activity
1. Fluorescent shift assay for APOBECs RNA editing
Xiaojiang S. Chen
2. Low Error Sequencing Methods to Tetect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System
Xiaojiang S. Chen
3. "Safe-Barcode" RNAseq assay for APOBECs RNA editing
Xiaojiang S. Chen
4. DT40 cell system to characterize somatic hypermutation
Silvestro Conticello
5. CH12 cell system to assay AID activity on class switch recombination
Silvestro Conticello
6. Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System
Linda Chelico
7. In vitro deamination assay to measure the activity and processivity of AID/APOBEC deaminases
Linda Chelico
8. Defining genome-wide mutagenic impact of APOBEC3 enzymes
Abby M. Green and Dávid Szüts
9. APOBEC-induced mutational assay in yeast
Steven Andrew Roberts

Section 2: Assays for APOBEC drug discovery
10. Biochemical assay for the identification of APOBECs inhibitors
Mani Larijani

Section 3: monitoring RNA or DNA editing
11. An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA
Rémi Buisson
12. Profiling rare C-to-U editing events via direct RNA sequencing
Ernesto Picardi and Adriano Fonzino
13. Global quantification of off-target activity by base editors
Erez Levanon and Michelle Eidelman

Section 4: Deaminases as tools for programmable RNA or DNA base editing
14. Programmable C to U RNA editing based on the APOBEC1 deaminase
Toshifumi Tsukahara

Section 5: Deaminases as tools for molecules tracking
15. Harnessing APOBEC editing for detecting RBP-RNA interactions
Gene W. Yeo and Tao Yu
16. Programmable C to U editing to track endogenous proteins
Tao Liu