Chemical Proteomics

Methods and Protocols
2012
ISBN: 978-1-61779-363-9
Verlag: Humana Press

Buch, Englisch, Band 803, 313 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 816 g

Reihe: Methods in Molecular Biology

The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.  In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chemical Proteomics: Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Bantscheff, Marcus

Drewes, Gerard

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

Part I: Introduction
1. Mass Spectrometry-Based Chemoproteomic Approaches
Marcus Bantscheff
2. Chemical Proteomics in Drug Discovery
Gerard Drewes
Part II: Small Molecules and Probe Design
3. Compound Immobilization and Drug-Affinity Chromatography
Uwe Rix, Manuela Gridling, and Giulio Superti-Furga
4. Affinity-based Chemoproteomics with Small Molecule-peptide Conjugates
Chaitanya Saxena
5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine
Xia Ge and Daniel S. Sem
6. Probing Proteomes with Benzophenone Photoprobes
Akira Kawamura and Doina M. Mihai
7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles
Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler
8. Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger
Part III: Target Discovery and Target Validation
9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC
Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr
10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags
Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff
11. Affinity-Based Profiling of Dehydrogenase Subproteomes
Xia Ge and Daniel S. Sem
12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics
Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten
13. Fluorescence-Based Proteasome Activity Profiling
Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers
14. Chemical Cross-Linking and High Resolution Mass Spectrometry to Study Protein-Drug Interactions
Mathias Q. Müller and Andrea Sinz
15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry
Natalia Gasilova, Alexis Nazabal
16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies
Amy Hye Won Jeon, Gerold Schmitt-Ulms
Part IV: Ligand Discovery
17. Ligand Discovery Using Small-molecule Microarrays
Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler
18. Working with Small-Molecules-Preparing and Storing Stock Solutions and Determination of Kinetic Solubility
Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard
19.  A Database for Chemical Proteomics-ChEBI
Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck
20. Working with Small Molecules: Rules-of-Thumb of “Drug Likeness”
Ming-Qiang Zhang