Marine Genomics

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2016
ISBN: 978-1-4939-8135-9
Verlag: Springer

Buch, Englisch, Band 1452, 253 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 5018 g

Reihe: Methods in Molecular Biology

This volume presents the latest protocols for both laboratory and

bioinformatics based analyses in the field of marine genomics. The chapters

presented in the book cover a wide range of topics, including the sampling and

genomics of bacterial communities, DNA extraction in marine organisms,

high-throughput sequencing of whole mitochondrial genomes, phylogenomics, SNP

discovery, SNP-arrays for species identification, digital PCR-based

quantification methods, environment DNA for invasive species surveillance and

monitoring, microarrays for the detection of waterborne pathogens, DNA

barcoding of marine biodiversity, metabarcoding protocols for marine

eukaryotes, analytical protocols for the visualization of eukaryotic diversity,

and applications for genomic data to benthic indices for environmental

monitoring. Written in the highly successful Methods in Molecular Biology

series format, chapters include introduction to their respective topics, lists

of the necessary materials and reagents, step-by-step, readily reproducible

laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Marine Genomics: Methods and Protocols is a

valuable resource for researchers, students, and policy makers in the field of

marine biology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Bourlat, Sarah J.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften Genetik und Genomik (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Wirbeltiere (Vertebrata) Säugetiere (Mammalia)
• Naturwissenschaften Biowissenschaften Meeresbiologie

Inhaltsverzeichnis

Sampling
of Riverine or Marine Bacterial Communities in Remote Locations: From Field to Publication.- DNA Extraction Protocols for Whole Genome Sequencing
in Marine Organisms.- High-Throughput
Sequencing of Complete Mitochondrial Genomes.- Phylogenomics
Using Transcriptome Data.- SNP
Discovery Using Next Generation Transcriptomic Sequencing.- SNP-Arrays for Species Identification in
Salmonids.- The
Next Generation PCR-Based Quantification Method for Ambient Waters: Digital PCR.- Using Environmental DNA for Invasive Species
Surveillance and Monitoring.- Microarrays/DNA Chips for the Detection
of Waterborne Pathogens.- DNA Barcoding of Marine Metazoans.- DNA Barcoding Marine Biodiversity: Steps
from Mere Cataloguing to Giving Reasons for Biological Differences.- Metabarcoding Marine Sediments: Preparation of Amplicon Libraries.- Preparation of Amplicon Libraries for Metabarcoding
of Marine Eukaryotes Using Illumina MiSeq: The Dual-PCRMethod.- Preparation of Amplicon Libraries for Metabarcoding
of Marine Eukaryotes Using Illumina MiSeq: The Adaptor-Ligation Method.- Visualizing Patterns of Marine Eukaryotic
Diversity from Metabarcoding Data Using QIIME.- Analysis
of Illumina MiSeq Metabarcoding Data: Application to Benthic Indices for Environmental
Monitoring.