Molecular Biology LabFax

Gene Analysis
2. Auflage 1998
ISBN: 978-0-12-136110-5
Verlag: William Andrew Publishing

Buch, Englisch, 255 Seiten, Format (B × H): 171 mm x 244 mm, Gewicht: 720 g

In the eight years since the original publication of Molecular Biology Labfax, there has been a vast proliferation of molecular biology techniques. The Second Edition has been divided into two parts: Recombinant DNA, and Gene Analysis. Together they comprise a comprehensive collection of the most up-to-date methods available in molecular biology. This second volume of the two-part Second Edition provides key information on nucleic acid blotting and hybridization, DNA sequencing, PCR, labelingnucleic acids, electrophoresis, centrifugation, chemicals and reagents, and safety. Molecular Biology Labfax will be essential for scientists of all disciplines within the life sciences who use molecular biological techniques.

Zielgruppe

AUDIENCE: Research scientists at the postgraduate, postdoctoral level and above working in molecular biology, recombinant DNA, gene cloning, as well as other areas within the life sciences.

Autoren/Hrsg.

Brown, T. A.

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Abbreviations.
Nucleic Acid Blotting and Hybridization:

Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
DNA Sequencing:

Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
Troubleshooting.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:

Amplification Methodology.
Cloning PCR Products.
Sequencing PCR Products.
Troubleshooting.
Labeling Nucleic Acids:

Radiochemistry.
Labeling Procedures.
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Non-Radioactive Labeling.
Agarose Gel Electrophoresis:

Agarose Gels.
Electrophoresis Systems.
DNA and RNA Markers for Agarose Gels.
Centrifugation:

Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:

Details of Individual Compounds.
Safety:

Protection from Chemical Hazards.
Protection from Microbiological Hazards.