Celiac Disease

Erscheinungsjahr 2023
ISBN: 978-0-443-19202-9
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 191 mm x 234 mm, Gewicht: 450 g

Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.

Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.

Autoren/Hrsg.

Weitere Mitwirkende

Castellanos-Rubio, Ainara

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Pepsin-trypsin digested gliadin treatment in intestinal cells
Ane Olazagoitia-Garmendia

2. Ex vivo gliadin stimulation of intestinal cells
Linda Zhang and Chuan He

3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers
Olga Biskou and Amaia Jauregi-Miguel

4. Intestinal permeability assessment using lactulose and mannitol in celiac disease
Silvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, and
Jose Ramon Bilbao Catala

5. In vivo sensitization to gliadin by oral administration
M. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero

6. Separation of epithelial and immune cells from biopsy samples
Irati Romero-Garmendia

7. Study and isolation of human intestinal dendritic cell and macrophage subsets
Elisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo

8. Elucidating the role of microbes in celiac disease through gnotobiotic modeling
Alberto Caminero, Elena F. Verdu, and Heather J. Galipeau

9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients
Federica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo

10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge
Concepcion Nuñez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Bañares, A. Bodas, S. Farrais,
N. Lopez-Palacios, and Mercedes Rubio

11. Isolation and cryopreservation of peripheral blood mononuclear cells
Sara Gomez-Aguililla, Concepcion Nuñez, Mercedes Rubio, and Maria Corzo

12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease
Concepcion Nuñez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy

13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin
Francesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi

14. Human intestinal organoid models for celiac disease research
Valeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri

15. Preparation of pepsin trypsin digested gliadin for stimulation experiments
Luis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio

16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs
Maialen Sebastian-delaCruz and Ainara Castellanos-Rubio

Über Autor(innen)

Castellanos-Rubio, Ainara
Ainara Castellanos-Rubio obtained her PhD on Genetics from the University of the Basque Country (Spain) in 2010. During her PhD, she studied gene expression alterations and genetic polymorphisms associated to Celiac Disease. She did a short term research stay in the University of Tampere (Finland) under the supervision of Dr. Marku Makki and Dr. Katri Lindfors where she used three dimensional cell cultures to describe different pathways involved in Celiac Disease development. On 2011 she joined the Laboratory of Dr. Sankar Ghosh in Columbia University (NY, USA) where she carried out her postdoctoral studies. During her postdoctoral training, she studied the implication of long noncoding RNAs (lncRNAs) in the immune and inflammatory response and she discovered and functionally characterized a novel lncRNA involved in the susceptibility to Celiac Disease. Dr. Castellanos-Rubio is an Ikerbasque Associate in the University of the Basque Country, where she leads her own group. She is interested in the involvement of noncoding RNAs in the pathogenesis of autoimmune and inflammatory diseases and studies the influence of disease associated SNPs in the functional disturbance of these RNAs. More recently, she has become interested on the epitranscriptomic alterations involved in different aspects of RNA regulation and her group studies how SNPs and environmental factors can alter these epitranscriptomic signals influencing the inflammatory response that finally evolves in disease development.