Cell-wide Metabolic Alterations Associated with Malignancy

Erscheinungsjahr 2014
ISBN: 978-0-12-801329-8
Verlag: William Andrew Publishing

Buch, Englisch, 384 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 840 g

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as "yet another" general hallmark of cancer, accumulating evidence suggests that the metabolic alterations of each neoplasm rather represent a molecular signature that intimately accompanies, and hence cannot be severed from, all facets of malignant transformation.

Autoren/Hrsg.

Weitere Mitwirkende

Kroemer, Guido

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Methods to measure cytoplasmic and mitochondrial Ca2+ concentration using Ca2+-sensitive dyes2. Methods to measure intracellular Ca2+ fluxes with Organelle-Targeted aequorin-based probes3. Methods to measure baseline Ca2+ levels with second-generation organelle-targeted fluorescent probes4. Methods to assess the autophagic flux in malignant cells5. Methods to assess autophagy in situ-transmission electron microscopy versus immunohistochemistry6. Methods to measure the enzymatic activity of PI3Ks7. Luciferase-based reporter to monitor the transcriptional activity of the SIRT3 promoter8. Metabolomic profiling of cultured cancer cells9. Pulsed stable isotope-resolved metabolomic studies of cancer cells10. Single-cell imaging for the study of oncometabolism 11. Study of cellular oncometabolism via multi-dimensional protein identification technology12. In vivo quantitative proteomics for the study of oncometabolism13. Metabolomic profiling of neoplastic lesions in mice14. Metabolomic profiling of tumor-bearing mice15. Metabolomic studies of patient material by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy16. Analysis of metabolomic profiling data acquired on GC-MS

Über Autor(innen)

Kroemer, Guido
Guido Kroemer got his M.D. in 1985 from the University of Innsbruck, Austria, and his Ph.D. in molecular biology in 1992 from the Autonomous University of Madrid, Spain. He is currently Professor at the Faculty of Medicine of the University of Paris Descartes/Paris V, Director of the INSERM research team 'Apoptosis, Cancer and Immunity', Director of the Metabolomics and Cell Biology platforms of the Gustave Roussy Cancer Campus, and Practitioner at the Hôpital Européen George Pompidou (Paris, France). He is also the Director of the Paris Alliance of Cancer Research Institutes (PACRI) and the Labex 'Immuno-Oncology'. Dr. Kroemer is best known for the discoveries that mitochondrial membrane permeabilization constitutes a decisive step in regulated cell death; that autophagy is a cytoprotective mechanism with lifespan-extending effects; and that anticancer therapies are successful only if they stimulate tumour-targeting immune responses. He is currently the most-cited cell biologist in Europe (relative to the period 2007-2013), and he has received the Descartes Prize of the European Union, the Carus Medal of the Leopoldina, the Dautrebande Prize of the Belgian Royal Academy of Medicine, the Léopold Griffuel Prize of the French Association for Cancer Research, the Mitjavile prize of the French National Academy of Medicine and a European Research Council Advanced Investigator Award.