Cilia: From Mechanisms to Disease-Part B

Erscheinungsjahr 2023
ISBN: 978-0-443-18588-5
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Cilia: From Mechanisms to Disease Part B, Volume 176 of the Methods in Cell Biology series, presents interesting chapters on topics such as The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish, Human LUHMES and NES cells as models for studying primary cilia in neurons, Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations, Single-molecule imaging in the primary cilium, Methods to analyze primary cilia in mouse cardiac lesion model, Methods to study motile ciliated cell types in a zebrafish brain mode, and more.

Other sections cover Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome), Modeling ciliopathies in patient-derived primary cells, Analysis of motility and mucociliary function of tracheal epithelial cilia, High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium, Methods for siRNA delivery in retina explants, Methods to study primary cilia and autophagy in the brain, and more.

Autoren/Hrsg.

Weitere Mitwirkende

Bravo-San Pedro, Jose Manuel

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish
Magdalena Cardenas Rodriguez and Iain Drummond
2. Human LUHMES and NES cells as models for studying primary cilia in neurons
Peter Swoboda
3. Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations
Russell J. Ferland
4. Single-molecule imaging in the primary cilium
Lucien E. Weiss
5. Methods to analyze primary cilia in mouse cardiac lesion model
Alfredo Criollo Sr., Daniel Peña-Oyarzun, Rodrigo Troncoso, Joseph A. Hill, Sergio Lavandero and Mauricio Budini Sr.
6. Methods to study motile ciliated cell types in a zebrafish brain mode
Nathalie Jurisch-Yaksi
7. Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome)
Diana Valverde
8. Modelling ciliopathies in patient-derived primary cells
Markus Schueler and Kai-Uwe Eckardt
9. Analysis of motility and mucociliary function of tracheal epithelial cilia
Koji Ikegami
10. High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium
Weidong Yang
11. Method for siRNA delivery in retina explants
Florent Poulhès
12. Methods to study primary cilia and autophagy in the brain
Olatz Pampliega
13. Multi-color live-cell fluorescence imaging of primary ciliary membrane assembly dynamics
Christopher Westlake

Über Autor(innen)

Bravo-San Pedro, Jose Manuel
Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).