Correlative Light and Electron Microscopy II

Erscheinungsjahr 2014
ISBN: 978-0-12-801075-4
Verlag: William Andrew Publishing

Buch, Englisch, 452 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 1180 g

This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

Autoren/Hrsg.

Weitere Mitwirkende

Muller-Reichert, Thomas

Verkade, Paul

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

1. Retracing in Correlative Light Electron Microscopy - Where is My Object of Interest? 2. Fluorescing the Electron: Strategies in Correlative Experimental Design 3. Metallothioneins for Correlative Light and Electron Microscopy 4. Correlative Light Microscopy and Electron Tomography to Study Von Willebrand Factor Exocytosis From Vascular Endothelial Cells 5. A Simple Procedure to Analyze Positions of Interest in Infectious Cell Cultures by Correlative Light and Electron Microscopy 6. Correlative Light and Electron Microscopy for a Free-Floating Spindle in Xenopuslaevis Egg Extracts   7. Combining Wide-field Super-resolution Microscopy and Electron Tomography: Rendering Nanoscopic Correlative Arrays on Subcellular Architecture 8. Correlative Cryo-fluorescence and Cryo-soft X-Ray Tomography of Adherent Cells at European Synchrotrons 9. Critical Step-by-step Approaches Toward Correlative Fluorescence/Soft X-Ray Cryo-Microscopy of Adherent Mammalian Cells 10. Correlative Cryo-fluorescence Light Microscopy and Cryo-electron Tomography of Streptomyces 11. Immuno Correlative Light and Electron Microscopy on Tokuyasu Cryosections 12. Step by Step Manipulation of the Cryocapsule with HPM High Pressure Freezers 13. Live-cell CLEM of Sub-cellular Targets: An Optimized Procedure for Polymer Based Imaging Substrates 14. Correlative Fluorescence- and Scanning Transmission Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid 15. The Use of a Laser for Correlating Light and Electron Microscopic Images in Thick Tissue Specimens 16. Correlative In Vivo 2-photon Imaging and Focused Ion Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure 17. Integrated Light and Scanning Electron Microscopy of GFP-Expressing Cells 18. Correlation of the Same Fields Imaged in the TEM, Confocal, LM and MicroCT by Image Registration: From Specimen Preparation to Displaying a Final Composite Image

Über Autor(innen)

Verkade, Paul
Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.

Muller-Reichert, Thomas
Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.

TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.
He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy.