CRISPR in Animals and Animal Models

Erscheinungsjahr 2017
ISBN: 978-0-12-812506-9
Verlag: William Andrew Publishing

Buch, Englisch, 148 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

CRISPR in Animals and Animal Models, Volume 152, the latest release in the Progress in Molecular Biology and Translational Science series, explores the genome editing CRISPR system in cells and animal models, its applications, the uses of the CRISPR system, and the past, present and future of CRISPR genome editing. Topics of interest in this updated volume include a section on CRISPR history, The genome editing revolution, Programming CRISPR and its applications, CRISPR Delivery methods, CRISPR libraries and screening, CRISPR investigation in haploid cells, CRISPR in the generation of transgenic animals, CRISPR therapeutics, and Promising strategies and present challenges.

Zielgruppe

<p>Scientists whose work is focussed on the generation cells and animals models of different human malignancies and also those involved in gene therapy approaches. </p>

Autoren/Hrsg.

Weitere Mitwirkende

Torres-Ruiz, Raul

Rodriguez-Perales, Sandra

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Gentherapie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien

Inhaltsverzeichnis

1. CRISPR History: Discovery, Characterization, and Prosperity Wenyuan Han and Qunxin She 2. CRISPR/Cas9 Technology: Applications and Human Disease Modeling Marta Martinez-Lage, Raúl Torres-Ruiz and Sandra Rodriguez-Perales 3. Dynamics of Indel Profiles Induced by Various CRISPR/Cas9 Delivery Methods Michael Kosicki, Sandeep Sundara Rajan, Flaminia C. Lorenzetti, Hans H. Wandall, Yoshiki Narimatsu, Emmanouil Metzakopian and Eric P. Bennett 4. CRISPR Libraries and Screening John T. Poirier 5. Genome Engineering Using Haploid Embryonic Stem Cells Takuro Horii and Izuho Hatada 6. CRISPR in Animals and Animal Models Ellen Shrock and Marc Guell 7. Gene Editing and CRISPR Therapeutics: Strategies Taught by Cell and Gene Therapy Juan C. Ramirez

Über Autor(innen)

Torres-Ruiz, Raul
Raúl Torres-Ruiz graduated in Biological Sciences (2004) and obtained his PhD in Molecular Biology (2013) for his studies related to genome engineering and viral vectors. His research work is based on the development of Genome Engineering novel technologies. This new technologies are characterized by a high potential in the study of causes and mechanisms involved in the development of human diseases. He has implemented the CRISPR-Cas9 system to reproduce chromosomal rearrangements characteristics of human neoplasias. One of the main objectives of our group was the development of methods and tools for human cell gene editing with therapeutic purposes and to model human illnesses. During this time, I have also work in the generation of novel systems for virus delivery (AAV, Adenovirus y Lentivirus) improving their potential as tools for basic and applied experimentation. In fact, one of those systems has been translated in a patent license.

Rodriguez-Perales, Sandra
Sandra Rodriguez-Perales graduated in Biological Sciences (1999) and obtained her PhD in Genetics (2004) for her studies on cancer related chromosomal translocations. After that period, she was awarded with a Postdoctoral Fellowship and joined the LMB-MRC (Cambridge, UK) during which period her interest centred on the induction of chromosomal translocations in animal models. In 2008, she joined as Scientific Researcher in the Molecular Cytogenetics Unit (CNIO, Madrid) applying genome engineering for the reproduction of chromosomal rearrangements and point mutations in human stem cells. Since 2008, she has been co-responsible for the cytogenetic diagnosis of hematologic malignancies and solid tumours and for providing extremely specialised, cutting edge technologies in molecular cytogenetics such as Spectral Karyotyping (SKY), fluorescence in situ hybridisation (FISH) for diagnosis and research, and array-based Comparative Genomic Hybridisation (aCGH) for the analysis of genomic aberrations. In 2008, she obtained the Clinical Cytogeneticist Licensure by the Spanish Association of Human Genetics (AEGH).