Flow Cytometry, Part A

2. Auflage 1994
ISBN: 978-0-12-203051-2
Verlag: Elsevier Science & Technology

Buch, Englisch, 591 Seiten

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

Autoren/Hrsg.

Darzynkiewicz, Zbigniew

Robinson, J. Paul

Crissman, Harry A.

Inhaltsverzeichnis

D.W. Visscher and J.D. Crissman, Dissociation of Intact Cells from Tumors and Normal Tissues.
Z. Darzynkiewicz, X. Li, and J. Gong, Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis.
C.C. Stewart and S.J Stewart, Cell Preparation for the Identification of Leukocytes.
C.C. Stewart and S.J. Stewart, Multiparameter Analysis of Leukocytes by Flow Cytometry.
G.F. Babcock and S.M. Dawes, Immunophenotyping Using Fixed Cells.
P.P.T.Brons, P.E.J. Van Erp, and A.H.M. Pennings, Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis.
R.A. Bray, Flow Cytometry Crossmatching for Solid Organ Transplantation.
H.M. Shapiro, Cell Membrane Potential Analysis.
M.J. Boyer and D.W. Hedley, Measurement of Intracellular pH.
C.H. June and P.S. Rabinovitch, Intracellular Ionized Calcium.
H.A. Crissman and J.A. Steinkamp, Cellular Protein Content Measurements.
F. Traganos and Z. Darzynkiewicz, Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations.
H.A. Crissman and G.T. Hirons, Staining of DNA in Live and Fixed Cells.
F.J. Otto, High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI.
L.L. Vindelov and I.J. Christensen, Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis.
D.W. Hedley, DNA Analysis from Paraffin-Embedded Blocks.
L.G. Dressler and L.C. Seamer, Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry.
P.S. Rabinovitch, DNA Content Histogram and Cell-Cycle Analysis.
F. Dolbeare and J.R. Selden, Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics.
J.A. Aten, J. Stap, R. Hoebe, and P.J.M. Bakker, Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers.
M. Poot, H. Hoehn, M. Kubbies, A. Grossmann, Y. Chen, and P.S. Rabinovitch, Cell-CycleAnalysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358(Ethidium Bromide Bivariate Flow Cytometry.
H.A. Crissman, N. Oishi, and R. Habbersett, Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods.
K.D. Bauer and J.W. Jacobberger, Analysis of Intracellular Proteins.
J.K. Larsen, Washless Procedures for Nuclear Antigen Detection.
W. Giaretti and M. Nusse, Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments.
Z. Darzynkiewicz, Simultaneous Analysis of Cellular RNA and DNA Content.
Z. Darzynkiewicz, J. Gong, and F. Traganos, Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell-Cycle.
J.P. Robinson, W.O. Carter, and P.K. Narayanan, Oxidative Product Formation Analysis by Flow Cytometry.
S. Klingel, G. Rothe, W. Kellermann, and G. Valet, Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates.
J.J. Turek and J.P. Robinson, Leucine Aminopeptidase Activity by Flow Cytometry.
J.V. Watson and C. Dive, Enzyme Kinetics.
K. Nooter, H. Herweijer, R.R. Jonker, and G.J. van den Engh, On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements.
Z. Darzynkiewicz, Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure.
J. Laffinand J.M. Lehman, Detection of Intracellular Virus and Viral Products.
Subject Index.

Über Autor(innen)

John P. Robinson is a Professor of Sociology at the University of Maryland, College Park. Dr. Robinson founded and directed the Survey Research Center at the University of Maryland and the Communication Research Center at Cleveland State University. He has published more than 100 articles in professional journals and books and is a contributing editor to American Demographics magazine. Dr. Robinson was an American Statistical Association/National Science Foundation fellow at the Bureau of Labor Statistics in 1992-93, a Fulbright scholar at Moscow State University and Soviet Academy of Sciences in 1990, a Research Consultant at BBC News in 1978 and Research Coordinator for the U.S. Surgeon General's Program on Television and Human Behavior in 1970. He received the 1987 Fordham University McGannon Award for Social and Ethical Relevance in Communication Policy Research for his research on improving public understanding of the news. His areas of specialization include social science methodology, attitude and behavior measurement, social change, and the impact of mass communication and other home technology. Additionally, he is an Editor of the Encyclopedia of Food Science, Food Technology & Nutrition.