Dietel / Wittekind / Bussolati Pre-Analytics of Pathological Specimens in Oncology

1. Auflage 2015
ISBN: 978-3-319-13957-9
Verlag: Springer International Publishing
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 199, 133 Seiten, eBook

Reihe: Recent Results in Cancer Research

This book presents an overview of the most important current developments in the pre-analytical handling of tissue. It addresses in particular potential ways to improve the situation whereby methods employed in the pre-analytical phase – the period from surgical removal of tissue to the start of pathological processing – have remained essentially unchanged for decades with only modest standardization. It is examined how the pre-analytical period can be optimized, resulting not only in an increase in diagnostic quality but also in a reduction in processing time and costs. Among the key topics examined are the so-called cold ischemia time between tissue removal and fixation, the potential superiority of vacuum-based preservation over immediate formalin fixation, two-temperature fixation, molecular analysis methods, and the pre-analytics of specimens from particular tissues. Readers will find this book to be an important update that reveals the full importance of the pre-analytical phase for quality of pathological work-up.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Dietel, Manfred

Wittekind, Christian

Bussolati, Gianni

Winterfeld, Moritz

Inhaltsverzeichnis

What Is Pre-Analytics in Surgical Pathology? .- Histologic Validation of Vacuum Sealed Formalin.- Free Tissue Preservation and Transport System .- Pre-Analytics and Molecular Pathology.- Experiences in Vacuum Preparation of Routine Specimens.- Tissue Heterogeneity as a Pre-Analytical Source of Variability.- Vacuum Fixation of Breast Specimens Before Grossing.- How Do Short Fixation and Rapid Microwave Processing Affect Her2 Testing?.- Current Projects in Pre-Analytics – Where to Go?.- Nucleic Acid Extraction from FFPE Tissue as Critical Preanalytic Step for NGS Applications.- Experiences With Pre-Analytics of Lung Specimen.- Experiences With Pre-Analytics of GI-Specimen.- Bone Marrow Work-Up.- Two-Temperature Fixation Preserves Activation Status with High Efficiency.- Towards the Lean Lab. The Industry Challenge.