Duque Environmental Responses in Plants

Methods and Protocols
1. Auflage 2016
ISBN: 978-1-4939-3356-3
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1398, 387 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues.

Environmental Responses in Plants: Methods and Protocols

is divided into four sections: Tropisms, Photoperiodism and Circadian Rhythms, Abiotic Stress Responses, and Plant-Pathogen Interactions. The chapters in these sections include detailed protocols to investigate some of the many key biological processes underlying plant environmental responses, mostly in the model organism

Arabidopsis thaliana,

but also in

Physcomitrella patens

and in different crop species such as rice, potato, barley, or tomato. Written in the highly successful

Methods in Molecular Biology

series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,

Environmental Responses in Plants: Methods and Protocols,

is a great resource for plant physiologists, biochemists, and cell and molecular scientists interested in this exciting and fast-growing research topic.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Duque, Paula

Inhaltsverzeichnis

Hydrotropism: Analysis of the Root Response to a Moisture Gradient.- Assessing Gravitropic Responses in A
rabidopsis.- 
Physiological Analysis of Phototropic Responses in
Arabidopsis.- A
utomatic Chloroplast Movement Analysis.- Microscopic and Biochemical Visualization of Auxins in Plant Tissues.- Immunolocalization of PIN and ABCB Transporters in Plants.- Analysis of Circadian Leaf Movements.- Sample Preparation of
Arabidopsis Thaliana
Shoot Apices for Expression Studies of Photoperiod-Induced Genes.- A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are Temporally Restricted by the Circadian Clock.- Identification of
Arabidopsis
Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome.-  Monitoring Alternative Splicing Changes in
Arabidopsis
Circadian Clock Genes.- Assessing the Impact of Photosynthetic Sugars on the
Arabidopsis
Circadian Clock.- Assessing Protein Stability Under Different Light and Circadian Conditions.- Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.- Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in
Arabidopsis Thaliana.- 
Assessing Tolerance to Heavy-Metal Stress in
Arabidopsis Thaliana
Seedlings.- Assessing Drought Responses Using Thermal Infrared Imaging.- Generating Targeted Gene Knockout Lines in
Physcomitrella Patens
to Study Evolution of Stress-Responsive Mechanisms.- Screening Stress Tolerance Traits in
Arabidopsis
Cell Cultures.- Using
Arabidopsis
Protoplasts to Study Cellular Responses to Environmental Stress.- Construction of Artificial miRNAs to Prevent Drought Stress in
Solanum Tuberosum.- 
Virus-Induced Gene Silencing for Gene Function Studies in Barley.- Methods for Long-Term Stable Storage of
Colletotrich
um Species.- Plant Inoculation With the Fungal Leaf Pathogen
Colletotrichum higginsianum.- 
Tracing Plant Defense Responses in Roots Upon MAMP/DAMP Treatment.- Analysis of the Immunity-Related Oxidative Bursts by a Luminol-Based Assay.- Quantitative Analysis of Microbe- Associated-Molecular-Pattern (MAMP)-Induced Ca
2+
Transients in Plants.- Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR.- Determining Nucleosome Position at Individual Loci after Biotic Stress Using MNase-qPCR.- Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in
Arabidopsis thaliana.

Über Autor(innen)

Paula Duque
Plant Molecular Biology group
Instituto Gulbenkian de Ciência
Rua da Quinta Grande, 6
2780-156 Oeiras
Portugal

Phone: [phone]
Fax: [phone]
Email: [e-mail]