Elzinga Methods in Protein Sequence Analysis

Erscheinungsjahr 2012
ISBN: 978-1-4612-5832-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 3, 589 Seiten, eBook

Reihe: Experimental Biology and Medicine

Methods in Protein Sequence Analysis contains an intensely prac tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se quence Analysis, which was held at Brookhaven National Labo ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas phase" sequencing.

Zielgruppe

Research

Autoren/Hrsg.

Elzinga, Marshall

Inhaltsverzeichnis

Conference Lecture.- Overview.- Strategy.- Improvements in Instrumentation and the Chemistry of Isothiocyanate Degradation.- Microsequencing.- Solid Phase Sequencing.- Manual Sequencing.- Sequencing by Mass Spectrometry.- Cleavage of Proteins.- Coordination of Protein and DNA Sequencing.- Use of High Performance Liquid Chromatography (HPLC) For the Separation of Proteins, Peptides, and Amino Acids.- Identification of Phenylthiohydantoins.- Communications: Sequence Methods.- Communications: New Sequences.