G4 biology

Erscheinungsjahr 2024
ISBN: 978-0-443-21774-6
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 610 g

G4 Biology, Volume 695 in the Methods in Enzymology series, highlights high value topics such as Assays to measure G4 resolution by DNA helicases, In gel staining methods of G4 DNA and RNA structures, Methods to probe the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolution, Analyzing the effect of transcription coupled R-loop formation on G4 formation, Integrated Structural Biology for the study of higher-order G4 structures, Advancing the Study of Protein-G4 Interactions in DNA Repair: Insights from Biolayer Interferometry and Circular Dichroism Spectroscopy, Immunofluorescence microscopy (IF) of G-quadruplex and R-loops, and more. Additional chapters delve into Production of the anti-G-quadruplex antibody BG4 for efficient ChIP-seq and CUT&Tag analysis: from plasmid quality control to validation, Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures, Potentiometric titrations to study ligand interactions with DNA i-motifs, Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR Spectroscopy, and Identification of bona fide G4-binding proteins, Observing Pif1 opening dsDNA and G4 in real time by manipulation under magnetic tweezers, and in vivo detection of DNA secondary structures using Permanganate/S1 Footprinting with direct adapter ligation and sequencing (PDAL-Seq).

Autoren/Hrsg.

Weitere Mitwirkende

Raney, Kevin D

Eoff, Robert

Byrd, Alicia

Kendrick, Samantha

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

1. Assays to measure G4 resolution by DNA helicases
Robert Brosh
2. In gel staining methods of G4 DNA and RNA structures
Katrin Paeschke
3. Methods to probe the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolution
Nasim Sabouri
4. Analyzing the effect of transcription coupled R-loop formation on G4 formation
Sua Myong
5. Integrated Structural Biology for the study of higher-order G4 structures
Jonathan Chaires
6. Advancing the Study of Protein-G4 Interactions in DNA Repair: Insights from Biolayer Interferometry and Circular Dichroism Spectroscopy
Colin Wu
7. Immunofluorescence microscopy (IF) of G-quadruplex and R-loops
Giovanni Capranico
8. Observing Pif1 opening dsDNA and G4 in real time by manipulation under magnetic tweezers
Jean-Baptiste Boulé
9. in vivo detection of DNA secondary structures using Permanganate/S1 Footprinting with direct adapter ligation and sequencing (PDAL-Seq)
Angelika Lahnsteiner
10. Production of the anti-G-quadruplex antibody BG4 for efficient ChIP-seq and CUT&Tag analysis: from plasmid quality control to validation
Sara Richter
11. Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures
Zoë Waller and Dilek Guneri
12. Potentiometric titrations to study ligand interactions with DNA i-motifs
Anton Granzhan
13. Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR Spectroscopy
Lukás Trantírek
14. Identification of bona fide G4-binding proteins
Pavel Ivanov