Microchip Capillary Electrophoresis

Methods and Protocols
1. Auflage. Softcover version of original hardcover Auflage 2006
ISBN: 978-1-61737-476-0
Verlag: Humana Press

Buch, Englisch, Band 339, 238 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 367 g

Reihe: Methods in Molecular Biology

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Zielgruppe

Research

Autoren/Hrsg.

Henry, Charles

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie
• Naturwissenschaften Chemie Physikalische Chemie Quantenchemie, Theoretische Chemie

Inhaltsverzeichnis

Microchip Capillary Electrophoresis.- Microchip Capillary Electrophoresis.- Microchip Fabrication Methods.- Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes.- Micro-Molding for Poly(dimethylsiloxane) Microchips.- Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation.- Surface Modification Methods.- Surface Modification Methods for Enhanced Device Efficacy and Function.- Polyelectrolyte Coatings for Microchip Capillary Electrophoresis.- Detection Methods for Microchip Capillary Electrophoresis.- Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry.- Interfacing Amperometric Detection With Microchip Capillary Electrophoresis.- Conductivity Detection on Microchips.- Applications of Microchip Capillary Electrophoresis.- DNA Separations.- Protein Separations.- Microchip Capillary Electrophoresis.- Measuring Electroosmotic Flow in Microchips and Capillaries.- Single Cell Analysis on Microfluidic Devices.- Rapid DNA Amplification in Glass Microdevices.