Post-Translational Modifications of Proteins

Tools for Functional Proteomics
2. Auflage. Softcover version of original hardcover Auflage 2008
ISBN: 978-1-61737-739-6
Verlag: Humana Press

Buch, Englisch, Band 446, 390 Seiten, Previously published in hardcover, Format (B × H): 156 mm x 234 mm, Gewicht: 572 g

Reihe: Methods in Molecular Biology

This is a fully up-dated and expanded practical guide to protein structure-function relationships. This important area of research is brought up-to-date by the leading scientists in the field. The compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology™ series. Comprehensive and cutting edge, the book serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Zielgruppe

Research

Autoren/Hrsg.

Kannicht, Christoph

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie

Inhaltsverzeichnis

Disulfide Bond Mapping by Cyanylation-induced Cleavage and Mass Spectrometry.- Detection of Post-translational Modifications by Fluorescent Staining of Two-Dimensional Gels.- Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods.- Analysis of Tyrosine-O-Sulfation.- ?-Amidated Peptides: Approaches for Analysis.- ?-Glutamate and ?–Hydroxyaspartate in Proteins.- Lysine Hydroxylation and Cross-linking of Collagen.- Mass Spectrometric Determination of Protein Ubiquitination.- Analysis of Sumoylation.- Detection and Analysis of Protein ISGylation.- Analysis of Methylation, Acetylation, and other Modifications in Bacterial Ribosomal Proteins.- Analysis of S-Acylation of Proteins.- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa.- 2-Dimensional Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern.- Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.- Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB).- Separation of N-Glycans by HPLC.- Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry – detection of Lewis X Structures.- Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins.- Analysis of O-Glycosylation.- Characterization of Site-specific N-Glycosylation.- Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.- Comparability and Monitoring Immunogenic N-linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines using HPLC with FluorescenceDetection and Mass Spectrometry.- Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-Linked Oligosaccharides of Recombinant Monoclonal Antibodies.- Web-based Computational Tools for the Prediction and Analysis of Post-translational Modifications of Proteins.