Buch, Englisch, 200 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 390 g
Buch, Englisch, 200 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 390 g
ISBN: 978-0-12-420070-8
Verlag: William Andrew Publishing
This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including:
- Pulse-chase analysis to measure protein degradation
- Labeling a protein with fluorophores using NHS ester derivitization
- Immunoaffinity purification of proteins
- Proteolytic affinity tag cleavage
- Purification of GST-tagged proteins
Zielgruppe
<p>Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists</p>
Fachgebiete
Weitere Infos & Material
Practical Steady-state Enzyme Kinetics
Quantification of Protein Concentration using UV absorbance and Coomassie Dyes
Preparation of protein samples for mass spectrometry and N-terminal sequencing
Lysis of mammalian and Sf9 cells
In Vivo [35 S]-Methionine Incorporation
Pulse-chase analysis to measure protein degradation
Labeling of a Protein with Fluorophores using Maleimide Derivitization
Labeling a protein with fluorophores using NHS ester derivitization
Protein Derivitization-Expressed Protein Ligation
Protein Biotinylation
Purification of His-tagged proteins
Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
Immunoaffinity purification of proteins
Affinity Purification of Protein Complexes Using TAP Tags
Strep-tagged protein purification
Proteolytic affinity tag cleavage
Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
Protein Affinity Purification using Intein/Chitin Binding Protein Tags
Purification of GST-tagged proteins