Laboratory Methods in Enzymology: Protein Part C

Erscheinungsjahr 2014
ISBN: 978-0-12-420119-4
Verlag: William Andrew Publishing

Buch, Englisch, 296 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 590 g

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Zielgruppe

<p>Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists</p>

Autoren/Hrsg.

Weitere Mitwirkende

Lorsch, Jon

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein-Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein-Nucleic Acid Interactions - Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates