Eicosanoid Protocols

1999
ISBN: 978-0-89603-667-3
Verlag: Humana Press

Buch, Englisch, Band 120, 296 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1020 g

Reihe: Methods in Molecular Biology

Since the pioneering work of U. S. VonEuler, G. O. Burr, B. Samuelsson, and others in the field of eicosanoids, research in this area continues to grow rapidly. Novel eicosanoids are being discovered even as enzymes that ca- lyze the synthesis of well-established eicosanoids are being critically studied with respect to their regulation and function. The novice in this field will most likely encounter three areas of intense research activity: regulation of expression and function of enzymes, i.e., ph- pholipases, cyclooxygenases, and lipoxygenases involved in the syntheses of established eicosanoids, characterization and distribution in tissues of eicosanoid receptors, and discovery and biologic roles of novel eicosanoids. This book is a compilation of chapters addressing these three areas. Most chapters of Eicosanoid Protocols address the first area, giving p- ticular emphasis to the cyclooxygenases and their two isoforms. This was done intentionally, because the discovery of the constitutive and inducible isoforms of this enzyme have introduced new concepts in the pathobiology of inflammation and in the use of nonsteroidal anti-inflammatory drugs. Although receptors of most established eicosanoids have been characterized and cloned, only one chapter (on the thromboxane A receptor) was devoted to this area.

Zielgruppe

Research

Autoren/Hrsg.

Lianos, Elias A

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Enzymologie
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Use of a Retroviral Vector for Expression of a Foreign Gene.- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues.- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization.- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin.- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography.- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry.- Assessment of Cyclooxygenase Protein Expression by Western Blotting.- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells.- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction.- Enzyme immunoassays (EIA) of Eicosanoids.- Transcellular Regulation of Eicosanoid Biosynthesis.- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry.- Epoxyeicosatrienoic Acids.- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts.- Assessment of 5-Lipoxygenase Activity and Cellular Distribution.- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids.- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition.- Novel Eicosanoids.