Buch, Englisch, Band 1421, 288 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 7172 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1421, 288 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 7172 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-3589-5
Verlag: Springer
This detailed volume explores the continuing techniques of
studying RNA-protein complexes and interactions as research in these areas
expand. After an introductory chapter,
the book continues with ways to purify RNA-protein complexes assembled in cells
or in isolated cellular extracts, methods for measuring various biochemical
activities of RNA-interacting proteins or ribonucleoproteins, biochemical
methods for measuring direct RNA-protein contact, as well as various new or
innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series,
chapters contain brief introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, RNA-Protein
Complexes and Interactions: Methods and Protocols provides a set of useful
protocols, both basic and advanced, designed to inspire researchers working
with RNA and RNA-interacting proteins.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Characterization of
RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and
SRSF2.- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA
Affinity Purification).- Biotin-Streptavidin Affinity Purification of
RNA-Protein Complexes Assembled In Vitro.- Detecting RNA-Protein Interaction
Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting.- Purification
of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing
Reaction Mixture.- Loading of Argonaute Protein with Small Duplex RNA in
Cellular Extracts.- In Vitro Analysis of Ribonucleoprotein Complex Remodeling
and Disassembly.- Single-Turnover Kinetics of Methyl Transfer to tRNA by
Methyltransferases.- Purification and Functional Reconstitution of Box H/ACA
Ribonucleoprotein Particles.- Northwestern Blot Analysis: Detecting RNA-Protein
Interaction After Gel Separation of Protein Mixture.- Measuring mRNA
Translation by Polysome Profiling.- Genome-Wide Profiling of RNA-Protein
Interactions Using CLIP-Seq.- Identification of Endogenous mRNA-Binding
Proteins in Yeast Using Crosslinking and PolyA Enrichment.- Ribo-Proteomics
Approach to Profile RNA-Protein and Protein-Protein Interaction Networks.- Detection
of Protein-Protein Interaction Within an RNA-Protein Complex Via
Unnatural-Amino-Acid-Mediated Photochemical Crosslinking.- Evolution of
Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.- mCarts: Genome-Wide
Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding
Proteins.- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in
Human Cells with Artificial Splicing Factors.- Informational Suppression to
Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5’ Splice-Site
Base-Pairing.- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina
Using a Fluorescent Reporter.
