Lin RNA-Protein Complexes and Interactions

Methods and Protocols
1. Auflage 2016
ISBN: 978-1-4939-3591-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1421, 288 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand.  After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, RNA-Protein Complexes and Interactions: Methods and Protocols provides a set of useful protocols, both basic and advanced, designed to inspire researchers working with RNA and RNA-interacting proteins.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Lin, Ren-Jang

Inhaltsverzeichnis

Characterization of
RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and
SRSF2.- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA
Affinity Purification).- Biotin-Streptavidin Affinity Purification of
RNA-Protein Complexes Assembled In Vitro.- Detecting RNA-Protein Interaction
Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting.- Purification
of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing
Reaction Mixture.- Loading of Argonaute Protein with Small Duplex RNA in
Cellular Extracts.- In Vitro Analysis of Ribonucleoprotein Complex Remodeling
and Disassembly.- Single-Turnover Kinetics of Methyl Transfer to tRNA by
Methyltransferases.- Purification and Functional Reconstitution of Box H/ACA
Ribonucleoprotein Particles.- Northwestern Blot Analysis: Detecting RNA-Protein
Interaction After Gel Separation of Protein Mixture.- Measuring mRNA
Translation by Polysome Profiling.- Genome-Wide Profiling of RNA-Protein
Interactions Using CLIP-Seq.- Identification of Endogenous mRNA-Binding
Proteins in Yeast Using Crosslinking and PolyA Enrichment.- Ribo-Proteomics
Approach to Profile RNA-Protein and Protein-Protein Interaction Networks.- Detection
of Protein-Protein Interaction Within an RNA-Protein Complex Via
Unnatural-Amino-Acid-Mediated Photochemical Crosslinking.- Evolution of
Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.- mCarts: Genome-Wide
Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding
Proteins.- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in
Human Cells with Artificial Splicing Factors.- Informational Suppression to
Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5’ Splice-Site
Base-Pairing.- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina
Using a Fluorescent Reporter.