Endocannabinoid Signaling

Methods and Protocols
1. Auflage 2016
ISBN: 978-1-4939-3537-6
Verlag: Springer

Buch, Englisch, Band 1412, 285 Seiten, HC runder Rücken kaschiert, Format (B × H): 183 mm x 260 mm, Gewicht: 7774 g

Reihe: Methods in Molecular Biology

This

volume encompasses all major methodologies to interrogate endocannabinoid

systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest

towards the manifold activities of eCBs, this book discusses the chemical,

biochemical, and molecular biological assays, and activity of distinct elements

of the ECS. These include membrane, nuclear receptors, biosynthetic and

hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in

the highly successful Methods in Molecular Biology series format,

chapters include introductions to their respective topics, lists of the

necessary materials and reagents, step-by-step, readily reproducible laboratory

protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocols

is a valuable resource and will help chemists, drug designers, biochemists,

molecular biologists, cell biologists, pharmacologists, and (electro)

physiologists navigate the mare magnum of endocannabinoid research.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Maccarrone, Mauro

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Need for Methods to Investigate
Endocannabinoid Signaling.- Extraction and Simultaneous Quantification
of Endocannabinoids and Endocannabinoid-Like Lipids
in Biological Tissues.- Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS.- Analysis of Omega-3 Fatty Acid
Derived
N
-acylethanolamines in Biological
Matrices.- Assay of CB
1
Receptor Binding.- The Displacement Binding Assay Using Human
Cannabinoid CB
2
Receptor-Transfected Cells.- Assay of TRPV1 Receptor Signaling.- A Functional Assay for GPR55: Envision Protocols.- The Cyclic AMP Assay Using Human Cannabinoid
CB
2
Receptor-Transfected Cells.- Assay of GTP?S Binding in Autoradiography.- Protocol to Study ß-Arrestin Recruitment by CB
1
and CB
2
Cannabinoid Receptors.- Assay of NAT Activity.- Assay of NAPE-PLD Activity.- Assay of FAAH Activity.- Assay of NAAA Activity.- Assay of DAGLa/ß Activity.- Assay of Monoacylglycerol Lipase Activity.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD6.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD12.-  Assay of Endocannabinoid Uptake.- Assay of Endocannabinoid Oxidation by Cyclooxygenase-2.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome P
450.-  
Assessing Gene Expression of the Endocannabinoid System.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking
in Cultured Cells.- Measuring ECS Interaction with Biomembranes.- Visualization of Endocannabinoids in the Cell.