Mach-Aigner / Martzy Trichoderma reesei

Methods and Protocols
Erscheinungsjahr 2020
ISBN: 978-1-0716-1048-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2234, 364 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus

Trichoderma reesei

. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful

Methods in Molecular Biology

series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical,

Trichoderma reesei: Methods and Protocols

serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Mach-Aigner, Astrid R.

Martzy, Roland

Inhaltsverzeichnis

Ecological Genomics and Evolution of
Trichoderma reesei
.- Industrial Relevance of
Trichoderma reesei
as an Enzyme Producer.- The Potential of Synthetic Biology

for
Trichoderma reesei
.- Resistance Marker- and Gene Gun-Mediated Transformation of
Trichoderma reesei
.- Use of Auxotrophic Markers for Targeted Gene Insertions in
Trichoderma reesei
.- Open the Pores: Electroporation for the Transformation of
Trichoderma reesei
.- Sexual Crossing of
Trichoderma reesei
.- CRISPR/Cas9-Mediated Genome Editing of
Trichoderma reesei
.- The Copper-Controlled RNA Interference System in
Trichoderma reesei
.- Batch Cultivation of
Trichoderma reesei
.- Image Analysis Method for the Characterization of
Trichoderma reesei
during Fermentations.- Measurement of Cellulase and Xylanase Activities in
Trichoderma reesei
.-  Flow Cytometry for Filamentous Fungi.- Molecular Identification of
Trichoderma reesei
.-In Vivo Footprinting Analysis in
Trichoderma reesei
.- RNA Characterization in
Trichoderma reesei
.- Proteomic Profiling of the Secretome of 
Trichoderma reesei
.- Transcriptomics in
Trichoderma reesei
.- The Comprehensive and Reliable Detection of Secondary Metabolites in
Trichoderma reesei
: A Tool for the Discovery of Novel Substances.- In Silico Gene Analysis and Oligonucleotide Design for Construction

of Expression Vectors.- PacBio Long-Read Sequencing, Assembly, and
Funannotate
Reannotation of the Complete Genome of
Trichoderma reesei
QM6a.- TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations.