Maramorosch / Koprowski Methods in Virology

Volume VIII
1. Auflage 2014
ISBN: 978-1-4832-5901-7
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 414 Seiten, Web PDF

Methods in Virology, Volume VIII focuses on the methods used in virology, including microscopy, hybridization, viruses, and fingerprint analysis. The selection first offers information on the hybridization of viral nucleic acids; applications of oligonucleotide fingerprinting to the identification of viruses; and immunosorbent electron microscopy in plant virus studies. Discussions focus on the detection of double-stranded RNA, principles and mechanisms of fingerprint analysis, preparation of labeled nucleic acid probes, and basic methods of nucleic acid hybridization. The text then elaborates on quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses and use of thin sectioning for visualization and identification of plant viruses. Topics include technical procedures for processing plant tissues, cytological modifications of diagnostic value, procedure and treatment of data to obtain the average mass of virus particles, and applications in virology. The book takes a look at the detection of genome-linked proteins of plant and animal viruses; methods for assay, purification, and characterization of prions; and the use of mosquitoes to detect and propagate viruses. The selection is a valuable source of information for researchers interested in the methods employed in virology.

Autoren/Hrsg.

Maramorosch, Karl

Koprowski, Hilary

Inhaltsverzeichnis

1;Front Cover;1
2;Methods in Virology;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors;10
6;Preface;12
7;Contents of Previous Volumes;14
8;Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution;20
8.1;I. Introduction;20
8.2;II. Purification of Nucleic Acid;22
8.3;III. Preparation of Labeled Nucleic Acid Probes;26
8.4;IV. Basic Methods of Nucleic Acid Hybridization;29
8.5;V. Southern Blot Hybridization;42
8.6;VI. Northern Blots;56
8.7;REFERENCES;57
9;Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses;60
9.1;I. Introduction;60
9.2;II. Principles and Mechanisms of Fingerprint Analysis;62
9.3;III. Experimental Procedures;68
9.4;IV. Applications;91
9.5;V. Summary and Conclusions;98
9.6;ACKNOWLEDGMENTS;99
9.7;REFERENCES;99
10;Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies;104
10.1;I. Introduction;104
10.2;II. Parameters of the Method;106
10.3;III. Varying Success with ISEM;115
10.4;IV. Applications;115
10.5;V. Detection of Double-Stranded RNA;117
10.6;VI. Preparation of Grids for Later Use;117
10.7;VII. Problems and Prospects for the Future;118
10.8;REFERENCES;119
11;Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass–Molecular Weight of Viruses;122
11.1;I. Introduction;122
11.2;II. Theory;124
11.3;III. Procedure and Treatment of Data to Obtain the Average Mass of Virus Particles;136
11.4;IV. Applications in Virology;142
11.5;V. Discussion;152
11.6;VI. Summary;157
11.7;ACKNOWLEDGMENTS;158
11.8;REFERENCES;158
12;Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses;162
12.1;I. Introduction;162
12.2;II. Technical Procedures for Processing Plant Tissues;163
12.3;III. Cytological Modifications of Diagnostic Value;197
12.4;ACKNOWLEDGMENTS;235
12.5;REFERENCES;235
13;Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion Bodies;244
13.1;I. Introduction;244
13.2;II. Inclusion Purification;245
13.3;III. Serological Analyses;273
13.4;IV. Discussion;296
13.5;ACKNOWLEDGMENTS;297
13.6;REFERENCES;297
14;Chapter 7. Use of Mosquitoes to Detect and Propagate Viruses;300
14.1;I. Rationale for Use of the Technique;300
14.2;II. Technical Considerations;301
14.3;III. Possible Limitations and Pitfalls;309
14.4;REFERENCES;311
15;Chapter 8. Prions: Methods for Assay, Purification, and Characterization;312
15.1;I. Introduction;313
15.2;II. Assays for Scrapie Prions;314
15.3;III. Histopathology;321
15.4;IV. Purification;323
15.5;V. Identification and Detection of PrP;335
15.6;VI. Electron Microscopy;345
15.7;VII. Polarization Microscopy;351
15.8;VIII. Biocontainment and Decontamination Procedures;353
15.9;ACKNOWLEDGMENTS;362
15.10;REFERENCES;362
16;Chapter 9. Detection of Genome-Linked Proteins of Plant and Animal Viruses;366
16.1;I. Introduction;366
16.2;II. Detection of a Genome-Linked Protein (VPg);367
16.3;III. Characterization of Genome-Linked Proteins and Adjacent Nucleotide Sequences;379
16.4;IV. Biosynthesis of Genome-Linked Proteins;387
16.5;V. Possible Functions of Genome-Linked Proteins;389
16.6;REFERENCES;395
17;Index;400