Buch, Englisch, Band 712, 295 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 590 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 712, 295 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 590 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-5755-2
Verlag: Humana Press
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression.- Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function.- Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag.- Biochemical Purification of Native Immune Protein Complexes.- Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity.- Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis.- Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis.- Constructing Haustorium-Specific cDNA Libraries from Rust Fungi.- Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes.- Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains.- A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores.- Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis.- Assaying Effector Function In planta Using Double-Barreled Particle Bombardment.- Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast.- Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana.- Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue.- Immunolocalization of Pathogen Effectors.- Laser Capture Microdissection of Nematode Feeding Cells.- Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling.- Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites.- Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins.