Measurement and Manipulation of Intracellular Ions

Erscheinungsjahr 1995
ISBN: 978-0-12-185297-9
Verlag: William Andrew Publishing

Buch, Englisch, 400 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 930 g

This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.

Autoren/Hrsg.

Weitere Mitwirkende

Conn, P. Michael

Kracier, Jacob

Dixon, S. Jeffrey

Fachgebiete

• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Physik Angewandte Physik Biophysik

Inhaltsverzeichnis

Light-Optical-Based Reagents for the Measurement and Manipulation of Ions, Metabolites, and Macromolecules in Living Cells, K.A. Giuliano and D.L. Taylor.
Use of Ion-Selective Microelectrodes for Recording Intracellular Ion Levels, M.E. Morris.
Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations, S.J. Karlik.
Use of Ionophores for Manipulating Intracellular Ion Concentrations, G.A. Woolley, D.R. Pfeiffer, and C.M. Deber.
Fluorescence Imaging of Cytosolic Calcium: An Introduction to Basic Experimental Principles, D.A. Williams.
Fluorescence Measurements of Cytosolic Calcium: Combined Photometry with Electrophysiology, W.T. Mason, J. Dempster, R. Zorec, J. Joyland, and P.M. Lledo.
Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium, A.M. Gurney and S.E. Bates.
Measurement of Cytosolic Calcium by 19 NMR, G.A. Smith and H.S. Bachelard.
Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes, S.J. Dixon and J.X. Wilson.
Brain Cell Acid-Base Changes Measured with Ion-Selective Microelectrodes, C.D. Lascola and R.P. Kraig.
Measurement of Cytosolic pH by Nuclear Magnetic Resonance Spectroscopy, O.A.C. Petroff and J.W. Prichard.
Manipulation and Regulation of Cytosolic pH, M.O. Bevensee and W.F. Boron.
Fluorescence Measurements of Cytosolic Sodium Concentration, M.A. Robertson and J.K. Foskett.
Measurement of Cytosolic Sodium Using Ion-Selective Microelectrodes, J.W. Deitmer and T. Munsch.
A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic Free Magnesium, E. Murphy and R.E. London.
Measurement of Cytosolic Magnesium by 31P NMR Spectroscopy, J.A. Helpern and H.R. Halvorson.
Chloride-Sensitive Fluorescent Indicators, A.S. Verkman and J. Biwersi.
Measurement of Cytosolic Chloride Activity by Ion-Selective Microelectrodes, J.A. Coles.
Use of Ion-Selective Microelectrodes and Fluorescent Probes to Measure Cell Volume, F.J. Alvarez-Leefmans, J. Altamirano, and W.E. Crowe.
Index.

Über Autor(innen)

Conn, P. Michael
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.