Methods for Monitoring Mitochondrial Functions During Pathogen Infection

Erscheinungsjahr 2025
ISBN: 978-0-323-99219-0
Verlag: William Andrew Publishing

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 188 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.

Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more.

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer
Toshio Miki
2. Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila
Pedro Escoll
3. Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis
Guochang Hu
4. Quantification of mitochondrial reactive oxygen species in macrophages during sepsis
Guochang Hu
5. Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response
Guochang Hu
6. Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection
Saverio Marchi
7. Monitoring mitochondria dynamics upon infection using a tomographic approach
Gianluca Morroni
8. A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells
Oliver Kepp
9. Isolation of mitochondria from Mycobacteria infected cells
Krishnaveni Mohareer
10. Monitoring mitochondrial localization of Mycobacterial proteins
Krishnaveni Mohareer
11. Bacterial predators and BALOs: growth protocol and relation with mithochondria
Valerio Iebba
12. Assessment of adhering and invading properties of Escherichia coli strains
Valerio Iebba
13. Bioinformatic techniques to study intracellular bacteria
Valerio Iebba