Micropatterning in Cell Biology, Part C

Micropatterning in Cell Biology
Erscheinungsjahr 2014
ISBN: 978-0-12-800281-0
Verlag: William Andrew Publishing

Buch, Englisch, 280 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 920 g

This new volume of Methods in Cell Biology looks at micropatterning in cell biology and includes chapters on protein photo-patterning on PEG with benzophenone, laser-directed cell printing and dip pen nanolithography. The cutting-edge material in this comprehensive collection is intended to guide researchers for years to come.

Zielgruppe

<p>Researchers and students in cell, molecular and developmental biology</p>

Autoren/Hrsg.

Weitere Mitwirkende

Théry, Manuel

Piel, Matthieu

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Section 1 Micropatterning soft substrates 1 Preparation of a Micropatterned Rigid-Soft Composite Substrate for Probing Cellular Rigidity Sensing 2 Patterned Hydrogels for Simplified Measurement of Cell Traction Forces 3 Micropatterning Hydroxy-PAAm Hydrogels and Sylgard 184 Silicone Elastomers with Tunable Elastic Moduli 4 The Facile Generation of 2-dimensional Stiffness Maps in Durotactic Cell Platforms Through Thickness Projections of 3-dimensional Submerged Topography 5 Micropatterning on Micropost Arrays 6 Development of Micropatterned Cell-Sensing Surfaces 7 Microfluidic Patterning of Protein Gradients on Biomimetic Hydrogel Substrates

Section 2 Cell and tissue micropatterning in 3D 8 Micropatterning of Poly(ethylene glycol) Diacrylate Hydrogels 9 Curved and Folded Micropatterns in 3D Cell Culture and Tissue Engineering 10 Cell Migration in Confined Environments 11 Micro-patterned Porous Membranes for Combinatorial Cell-based Assays 12 Micropatterning Cells on Permeable Membrane Filters 13 Microfabrication of a Platform to Measure and Manipulate the Mechanics of Engineered Microtissues 14 Methods for 2D Cell Confinement 15 Benzophenone-based Photochemical Micropatterning of Biomolecules to Create Model Substrates and Instructive Biomaterials

Über Autor(innen)

Piel, Matthieu
Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell's environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.