Helicobacter pylori Protocols

1. Auflage. Softcover version of original hardcover Auflage 1997
ISBN: 978-1-61737-020-5
Verlag: Humana Press

Buch, Englisch, Band 8, 274 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 446 g

Reihe: Methods in Molecular Medicine

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Mobley, Harry L. T.

Clayton, Christopher L.

Fachgebiete

• Naturwissenschaften Biowissenschaften Bakteriologie
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

Introduction: Medical Significance of H. pylori.- Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture.- Serology and Urea Breath Test in the Diagnosis of H. pylori Infection.- The Detection of H. pylori by the Polymerase Chain Reaction.- PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples.- In vitro Susceptibility Testing of H. pylori.- Defined Media for H. pylori.- Studies of Respiration in H. pylori.- Use of NMR to Study H. pylori Metabolism.- Isolation of H. pylori Genomic DNA and Restriction Analysis.- Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori.- Differentiation of H. pylori Strains Using PCR RFLP.- PCR-DNA Sequence Typing.- H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method.- Molecular Methods for Detecting Ulcerogenic Strains of H. pylori.- H. pylori DNA Transformation by Natural Competence and Electroporation.- Random Mutagenesis of the H. pylori Genome.- Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE).- Molecular Characterization of H. pylori Surface Antigens.- Isolation and Characterization of Porins from H. pylori.- Methods for the Identification of H. pylori Host Receptors.- Host Responses to H. pylori.- Nonprimate Animal Models of H. pylori Infection.- The Nonhuman Primate Model for H. pylori Infection.