Otvos Peptide-Based Drug Design

Erscheinungsjahr 2008
ISBN: 978-1-59745-419-3
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 494, 303 Seiten, eBook

Reihe: Methods in Molecular Biology

Due to their high specificity and low toxicity profile, peptides have once again become central to the development of new drugs. In

Peptide-Based Drug Design: Methods and Protocols

, expert researchers provide a handbook which offers a selection of research and production tools suitable for transforming a promising protein fragment or stand-alone native peptide into a pharmaceutically acceptable composition. The volume delves into contemporary, cutting-edge subjects such as hit isolation and target validation, computer-aided design, sequence modifications to satisfy pharmacologists, in vivo stability and imaging, and the actual production of difficult sequences. Written in the highly successful

Methods in Molecular Biology™

series format, chapters include readily reproducible, step-by-step laboratory protocols, lists of materials, and the Notes section, which highlights tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date,

Peptide-Based Drug Design: Methods and Protocols

shows its subject to be an independent science on the rise, and provides scientists with a clear, concise guide for continuing this vital research.

Zielgruppe

Research

Autoren/Hrsg.

Otvos, Laszlo

Inhaltsverzeichnis

Peptide-Based Drug Design: Here and Now.- Strategies for the Discovery, Isolation, and Characterization of Natural Bioactive Peptides from the Immune System of Invertebrates.- Sequence Analysis of Antimicrobial Peptides by Tandem Mass Spectrometry.- The Spot Technique: Synthesis and Screening of Peptide Macroarrays on Cellulose Membranes.- Analysis of A? Interactions Using ProteinChip Technology.- NMR in Peptide Drug Development.- Molecular Dynamics Simulations of Peptides.- Short Linear Cationic Antimicrobial Peptides: Screening, Optimizing, and Prediction.- Investigating the Mode of Action of Proline-Rich Antimicrobial Peptides Using a Genetic Approach: A Tool to Identify New Bacterial Targets Amenable to the Design of Novel Antibiotics.- Serum Stability of Peptides.- Preparation of Glycosylated Amino Acids Suitable for Fmoc Solid-Phase Assembly.- Synthesis of O-Phosphopeptides on Solid Phase.- Peptidomimetics: Fmoc Solid-Phase Pseudopeptide Synthesis.- Synthesis of Toll-Like Receptor-2 Targeting Lipopeptides as Self-Adjuvanting Vaccines.- Synthesis of a Multivalent, Multiepitope Vaccine Construct.- Cysteine-Containing Fusion Tag for Site-Specific Conjugation of Therapeutic and Imaging Agents to Targeting Proteins.