Paddock Confocal Microscopy

Methods and Protocols
Erscheinungsjahr 2008
ISBN: 978-1-59259-722-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 122, 446 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Research

Autoren/Hrsg.

Paddock, Stephen W.

Inhaltsverzeichnis

An Introduction to Confocal Imaging.- Practical Considerations for Collecting Confocal Images.- Fluorescent Probes for Confocal Microscopy.- Imaging Gene Expression Using Antibody Probes.- Single and Double FISH Protocols for Drosophila.- Confocal Microscopy of Plant Cells.- Preparation of Yeast Cells for Confocal Microscopy.- Confocal Methods for Caenorhabditis elegans.- Imaging Sea Urchin Fertilization.- Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy.- Confocal Microscopy on Xenopus laevis Oocytes and Embryos.- Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos.- Confocal Imaging of Living Cells in Intact Embryos.- Live Confocal Analysis with Fluorescently Labeled Proteins.- Live Imaging with Green Fluorescent Protein.- Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging.- Intracellular pH and pCa Measurement.- Measuring Dynamic Cell Volume In Situ by Confocal Microscopy.- Imaging Thick Tissues with Confocal Microscopy.- Measurement in the Confocal Microscope.- Presentation of Confocal Images.- The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides.- Information Management of Confocal Microscopy Images.- Morphing Confocal Images and Digital Movie Production.