Environmental Microbiology

A Laboratory Manual
Erscheinungsjahr 1995
ISBN: 978-0-12-550655-7
Verlag: Elsevier Science & Technology

Buch, Englisch, 175 Seiten, Format (B × H): 216 mm x 279 mm

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology.
The first section includes experiments on soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens -- bacteria, viruses, and protozoan parasites -- used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation.
Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Autoren/Hrsg.

Pepper, Ian L.

Gerba, Charles P.

Brendecke, Jeffrey W.

Fachgebiete

• Interdisziplinäres Bibliothekswesen, Informationswissenschaften Bibliotheks- und Auskunftssdienste

Inhaltsverzeichnis

Preface.
Soil Microbiology:
Moisture Content Determination.
Contact Slide Assay.
Filamentous Fungi.
Bacteria and Actinomycetes.
Algae: Enumerated by MPN.
Oxidation of Sulfur in Soil.
Dehydrogenase Activity of Soils.
Nitrification and Denitrification.
Water Microbiology:
Bacteriological Examination of Water: The Coliform MPN Test.
Membrane Filter Technique.
Detection of Bacteriophages.
Assimilable Organic Carbon.
New Methods for the Detection of Coliforms and Fecal Coliforms.
Dry Medium for the Detection of Coliforms in Water, Food, and on Surfaces.
Detection of Enteric Viruses in Water.
Detection of Waterborne Parasites.
Biodegradation of Phenol Compounds.
Environmental Biotechnology:
Molecular Detection of Microbial Pathogens Using Polymerase Chain Reaction.
Arbitrarily Primed PCR.
Enrichment Culture and BIOLOG.
Appendices:
Significant Figures.
Soil pH Determination.
Instrumental Molecular Absorbtion Spectrophotometry and Turbidimetry.
Glossary.

Über Autor(innen)

Ian L. Pepper is currently the Chair of the Undergraduate Program in Environmental Sciences at the University of Arizona. He was recently named to Who's Who in America for the second year in a row, and in 1994 earned the Researcher of the Year Award in the College of Agriculture at the University of Arizona. In 1994 he was also named as a fellow to the American Society of Agronomy, and he became the Chair of the Soil Biology and Biohemistry Division of the Soil Science Society of America. Pepper is also a Fellow of the American Society of Microbiology, and has been a member of the Editorial Board of Applied and Environmental Microbiology for the past nine years. Pepper has also been the author or co-author of numerous published journal articles.